Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F13%3A43885204" target="_blank" >RIV/60076658:12520/13:43885204 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0011224012002738" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0011224012002738</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2012.12.003" target="_blank" >10.1016/j.cryobiol.2012.12.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa

Popis výsledku v původním jazyce

Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaClsolutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compa

Název v anglickém jazyce

Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa

Popis výsledku anglicky

Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaClsolutions of differing osmolalities, ranging from 100 to 300 mOsm kg(-1) for 10 s, after which final osmolality was adjusted to 300 mOsm kg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200 mOsm kg(1) NaCl was significantly higher (44 +/- 10%) than in controls (21 +/- 15%) and samples pretreated with 100 mOsm kg(-1) (25 +/- 15%) and 300 mOsm kg(-1) (25 +/- 12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200 mOsm kg(-1) (119 +/- 24, 118 +/- 22, and 115 +/- 32 mu m s(-1), respectively) compa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryobiology

ISSN

0011-2240

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

192-194

Kód UT WoS článku

000316518100018

EID výsledku v databázi Scopus

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