Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888837" target="_blank" >RIV/60076658:12520/15:43888837 - isvavai.cz</a>

Výsledek na webu

<a href="http://lipidworld.biomedcentral.com/articles/10.1186/s12944-015-0159-2" target="_blank" >http://lipidworld.biomedcentral.com/articles/10.1186/s12944-015-0159-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12944-015-0159-2" target="_blank" >10.1186/s12944-015-0159-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro

Popis výsledku v původním jazyce

Background: Human hepatocellular cells Hep G2 were used to mimic and investigate the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, fatty acid (FA) and phospholipid class composition. Methods: Cells were incubated with a combination of Cd2+ and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) fish specific FA. Results: We measured a significant increased proportion of EPA and DHA in the treated cells compared to the control line confirming the uptake. While doses of25 mu M DHA showed to be toxic to the cells, repeated short term incubations (2 h) at lower doses resulted in an increased uptake of DHA. The resarzurin assay, evaluating cell viability, showed a significant decrease in cell viability between Cd2+ incubation time and, further, the pre-incubation with BSA-FA complex resulted in significantly increased cell viability. On the metabolic level, increased concentrations of EPA and DHA resulted in an increased proportion of docosapentaenoic aci

Název v anglickém jazyce

Combined incubation of Cadmium, docosahexaenoic and eicosapentaenoic acid results in increased uptake of cadmium and elevated docosapentaenoic acid content in Hepatocytes in vitro

Popis výsledku anglicky

Background: Human hepatocellular cells Hep G2 were used to mimic and investigate the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, fatty acid (FA) and phospholipid class composition. Methods: Cells were incubated with a combination of Cd2+ and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) fish specific FA. Results: We measured a significant increased proportion of EPA and DHA in the treated cells compared to the control line confirming the uptake. While doses of25 mu M DHA showed to be toxic to the cells, repeated short term incubations (2 h) at lower doses resulted in an increased uptake of DHA. The resarzurin assay, evaluating cell viability, showed a significant decrease in cell viability between Cd2+ incubation time and, further, the pre-incubation with BSA-FA complex resulted in significantly increased cell viability. On the metabolic level, increased concentrations of EPA and DHA resulted in an increased proportion of docosapentaenoic aci

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Lipids in Health and Disease

ISSN

1476-511X

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000365564200002

EID výsledku v databázi Scopus

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