Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43895410" target="_blank" >RIV/60076658:12520/17:43895410 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/17:00100458

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0887233317302199?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tiv.2017.08.003" target="_blank" >10.1016/j.tiv.2017.08.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro

Popis výsledku v původním jazyce

The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0?5 ?M) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 ?M cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium.

Název v anglickém jazyce

Effect of cadmium on uptake of iron, zinc and copper and mRNA expression of metallothioneins in HepG2 cells in vitro

Popis výsledku anglicky

The intake of cadmium contaminated fish was mimicked by incubating human hepatoblastoma cells (Cell line HepG2) with a combination of different levels of cadmium (0?5 ?M) plus the n-3 fatty acids docosahexaenoic acid and eicosapentaenoic acid, which are typical for fish. Uptake of cadmium, iron, copper and zinc was measured by ICP-MS. In addition mRNA expression of two metallothioneins (mt1 g and mt1 m) was evaluated by real-time PCR. The obtained data shows that the presence of cadmium increases the uptake of iron and zinc into the HepG2 cells while the uptake of copper remains unaffected. The presence of the chosen fatty acids did not affect the uptake of either cadmium or iron, zinc and copper. The presence of already 1 ?M cadmium increased the mRNA expression of mt1 g and mt1 m significantly, while the fatty acids did not interfere with the effect of cadmium.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology in vitro

ISSN

0887-2333

e-ISSN

—

Svazek periodika

44

Číslo periodika v rámci svazku

10/2017

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

372-376

Kód UT WoS článku

000409395000043

EID výsledku v databázi Scopus

2-s2.0-85028010599