Parametrization of discrete sphericity in electron microscopy images

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43896077" target="_blank" >RIV/60076658:12520/17:43896077 - isvavai.cz</a>

Výsledek na webu

<a href="http://www2.humusoft.cz/www/papers/tcp2017/036_urbanova.pdf" target="_blank" >http://www2.humusoft.cz/www/papers/tcp2017/036_urbanova.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Parametrization of discrete sphericity in electron microscopy images

Popis výsledku v původním jazyce

Electron microscopy allows us to observe nanostructures of the small objects inside the biological organisms (nuclei, photosysems, membranes). The obtained images are intensity (grayscale) levels, there is no color defined in the electrons beam. The image properties (contrast, bitdepth, dynamic range, resolution, ...) are person-specific, since the experts have an individual imaging-behavior. The background and type or size of other objects vary due to biological reasons. The aim of work is to discuss the sphericity attribute of several objects for classification purposes. Usually, the very small objects are also of small amount of occupied pixels, therefore the disretization of the object borders come into acount. The classicall Hough transformation is time consuming and valid for well spherical objects. However, in the electron microscopy several types of objects are of the experimentator interrest. Therefore, the detection and classification should be based on parametrization and evaluation of the sphericity attribute. The low time burden sollution could be done be using eliptical parameters, and area to perimeter derived ratio. The three types of electron microscopy images were tested, of different biological experiments. The parametrization of the classification criteria is automaticaly evaluated via statistical description of the given electron microscopy image.

Název v anglickém jazyce

Parametrization of discrete sphericity in electron microscopy images

Popis výsledku anglicky

Electron microscopy allows us to observe nanostructures of the small objects inside the biological organisms (nuclei, photosysems, membranes). The obtained images are intensity (grayscale) levels, there is no color defined in the electrons beam. The image properties (contrast, bitdepth, dynamic range, resolution, ...) are person-specific, since the experts have an individual imaging-behavior. The background and type or size of other objects vary due to biological reasons. The aim of work is to discuss the sphericity attribute of several objects for classification purposes. Usually, the very small objects are also of small amount of occupied pixels, therefore the disretization of the object borders come into acount. The classicall Hough transformation is time consuming and valid for well spherical objects. However, in the electron microscopy several types of objects are of the experimentator interrest. Therefore, the detection and classification should be based on parametrization and evaluation of the sphericity attribute. The low time burden sollution could be done be using eliptical parameters, and area to perimeter derived ratio. The three types of electron microscopy images were tested, of different biological experiments. The parametrization of the classification criteria is automaticaly evaluated via statistical description of the given electron microscopy image.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

24th Annual conference proceedings. Prague, Czech Republic

ISBN

978-80-7592-002-7

ISSN

2336-1662

e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

36

Název nakladatele

Vysoká škola chemicko technologická

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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