A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3AN0000001" target="_blank" >RIV/60076658:12520/20:N0000001 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000600344000008" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000600344000008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22092/IJFS.2020.122798" target="_blank" >10.22092/IJFS.2020.122798</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish

Popis výsledku v původním jazyce

Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish (Carassius auratus) were exposed to 1, 5, 25 mu g L-1 BPA and 1 mu g L-1 E2 for comparison over 24 h. Compared to control, the mRNA transcript of er beta-I showed a significant increase in the hepatic tissues exposed to all doses of BPA and E-2. The mRNA transcript of er beta-II was signifincantly increased in hepatic tissues exposed to 1 and 5 mu g L-1 BPA and E-2. The mRNA transcripts of vtg in the hepatic tissues treated with 5 mu g L-1 BPA and E-2 were also increased, significantly. Finally, cyp 1a mRNA transcript showed a significant increase in the hepatic tissues exposed to 5 and 25 mu g L-1 BPA and E-2. These results show an estrogenic activity of BPA similar to E-2, and suggest that in vitro approach can be used as an alternative to in vivo test to detect estrogenic effects of BPA.

Název v anglickém jazyce

A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish

Popis výsledku anglicky

Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish (Carassius auratus) were exposed to 1, 5, 25 mu g L-1 BPA and 1 mu g L-1 E2 for comparison over 24 h. Compared to control, the mRNA transcript of er beta-I showed a significant increase in the hepatic tissues exposed to all doses of BPA and E-2. The mRNA transcript of er beta-II was signifincantly increased in hepatic tissues exposed to 1 and 5 mu g L-1 BPA and E-2. The mRNA transcripts of vtg in the hepatic tissues treated with 5 mu g L-1 BPA and E-2 were also increased, significantly. Finally, cyp 1a mRNA transcript showed a significant increase in the hepatic tissues exposed to 5 and 25 mu g L-1 BPA and E-2. These results show an estrogenic activity of BPA similar to E-2, and suggest that in vitro approach can be used as an alternative to in vivo test to detect estrogenic effects of BPA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

—

Návaznosti

—

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

IRANIAN JOURNAL OF FISHERIES SCIENCES

ISSN

1562-2916

e-ISSN

1562-2916

Svazek periodika

19

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

IR - Íránská islámská republika

Počet stran výsledku

16

Strana od-do

2877-2892

Kód UT WoS článku

000600344000008

EID výsledku v databázi Scopus

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