Estimation of rheological parameters for unstained living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902667" target="_blank" >RIV/60076658:12520/21:43902667 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1140/epjs/s11734-021-00084-2" target="_blank" >https://doi.org/10.1140/epjs/s11734-021-00084-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1140/epjs/s11734-021-00084-2" target="_blank" >10.1140/epjs/s11734-021-00084-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of rheological parameters for unstained living cells

Popis výsledku v původním jazyce

In video-records, objects moving in intracellular regions are often hardly detectable and identifiable. To squeeze the information on the intracellular flows, we propose an automatic method of reconstruction of intracellular flow velocity fields based only on a recorded video of an unstained cell. The basis of the method is detection of speeded-up robust features (SURF) and assembling them into trajectories. Two components of motion-direct and Brownian-are separated by an original method based on minimum covariance estimation. The Brownian component gives a spatially resolved diffusion coefficient. The directed component yields a velocity field, and after fitting the vorticity equation, estimation of the spatially distributed effective viscosity. The method was applied to videos of a human osteoblast and a hepatocyte. The obtained parameters are in agreement with the literature data.

Název v anglickém jazyce

Estimation of rheological parameters for unstained living cells

Popis výsledku anglicky

In video-records, objects moving in intracellular regions are often hardly detectable and identifiable. To squeeze the information on the intracellular flows, we propose an automatic method of reconstruction of intracellular flow velocity fields based only on a recorded video of an unstained cell. The basis of the method is detection of speeded-up robust features (SURF) and assembling them into trajectories. Two components of motion-direct and Brownian-are separated by an original method based on minimum covariance estimation. The Brownian component gives a spatially resolved diffusion coefficient. The directed component yields a velocity field, and after fitting the vorticity equation, estimation of the spatially distributed effective viscosity. The method was applied to videos of a human osteoblast and a hepatocyte. The obtained parameters are in agreement with the literature data.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10305 - Fluids and plasma physics (including surface physics)

Projekt

<a href="/cs/project/LM2018099" target="_blank" >LM2018099: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Physical Journal Special Topics

ISSN

1951-6355

e-ISSN

1951-6401

Svazek periodika

230

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1105-1112

Kód UT WoS článku

000641242500002

EID výsledku v databázi Scopus

2-s2.0-85112120239