Motility and oxidative stress of common carp Cyprinus carpio sperm during short-term storage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904483" target="_blank" >RIV/60076658:12520/22:43904483 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10445274

Výsledek na webu

<a href="https://doi.org/10.1016/j.anireprosci.2022.106991" target="_blank" >https://doi.org/10.1016/j.anireprosci.2022.106991</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2022.106991" target="_blank" >10.1016/j.anireprosci.2022.106991</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Motility and oxidative stress of common carp Cyprinus carpio sperm during short-term storage

Popis výsledku v původním jazyce

Short-term storage of semen is a simple and inexpensive procedure to deal with logistics of large-scale hatchery operations but can lead to oxidative stress and a significant decrease in sperm motility and velocity. To better understand the mechanisms responsible for the association of poor sperm quality with oxidative stress, in the present study we investigated the effect of refrigerated storage for 0, 24, 48, 72, and 144 h on sperm motility and curvilinear velocity and oxidant/antioxidant balance of common carp Cyprinus carpio. Percentage of motile sperm was significantly (p &lt; 0.05) reduced stored for 72 h storage compared to that of fresh sperm. Sperm stored 144 h showed &lt; 40% motility with an average velocity of 91.12 +/- 10.4 mu m s(-1). A time dependent increase in the level of the oxidative stress indices lipid peroxidation and carbonyl derivatives of proteins was observed. Increase (p &gt; 0.05) in total superoxide dismutase was detected after 48 h, and glutathione reductase and glutathione peroxidase activity demonstrated a significant increase after 72 h. These results provide an additional tool for the development and improvement of short-term sperm preservation procedures commonly applied in aquaculture.

Název v anglickém jazyce

Motility and oxidative stress of common carp Cyprinus carpio sperm during short-term storage

Popis výsledku anglicky

Short-term storage of semen is a simple and inexpensive procedure to deal with logistics of large-scale hatchery operations but can lead to oxidative stress and a significant decrease in sperm motility and velocity. To better understand the mechanisms responsible for the association of poor sperm quality with oxidative stress, in the present study we investigated the effect of refrigerated storage for 0, 24, 48, 72, and 144 h on sperm motility and curvilinear velocity and oxidant/antioxidant balance of common carp Cyprinus carpio. Percentage of motile sperm was significantly (p &lt; 0.05) reduced stored for 72 h storage compared to that of fresh sperm. Sperm stored 144 h showed &lt; 40% motility with an average velocity of 91.12 +/- 10.4 mu m s(-1). A time dependent increase in the level of the oxidative stress indices lipid peroxidation and carbonyl derivatives of proteins was observed. Increase (p &gt; 0.05) in total superoxide dismutase was detected after 48 h, and glutathione reductase and glutathione peroxidase activity demonstrated a significant increase after 72 h. These results provide an additional tool for the development and improvement of short-term sperm preservation procedures commonly applied in aquaculture.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Svazek periodika

241

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000806560300005

EID výsledku v databázi Scopus

2-s2.0-85129922090