Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43904604" target="_blank" >RIV/60076658:12520/22:43904604 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3390/fishes7050248" target="_blank" >https://doi.org/10.3390/fishes7050248</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/fishes7050248" target="_blank" >10.3390/fishes7050248</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna

Popis výsledku v původním jazyce

The influence of water hardness on copper (Cu) toxicity in Daphnia magna was studied using gene expression analysis. Exposing D. magna to Cu in water with increasing levels of hardness decreased the acute toxicity. Hardness did not affect the predicted Cu complexation. After 24 h, D. magna showed an increased level of genes related to metal homeostasis (mt) following exposure to 25 mu g Cu/L in hard water. Daphnids in soft and medium water responded to 25 mu g Cu/L by upregulation of antioxidant defense and mt genes, revealing oxidative stress as a mechanism of Cu toxicity in D. magna. D. magna exposed to 25 mu g Cu/L in soft water did not survive for 96 h. In contrast, those exposed to 25 mu g Cu/L in medium and hard water survived for 96 h with significantly higher levels of mt genes. The genes related to oxidative damage (heat shock protein and glutathione S-transferase) in these groups did not deviate from control levels, indicating the protective effect of hardness. Metallothionein genes were upregulated at 17 mu g Cu/L at both 24 h and 96 h. The expression of catalase and ferritin increased in this group in soft and hard water at 96 h. The protective effect of hardness (in the tested range) on survival was also observed at a concentration of 25 mu g/L. The results suggest metallothionein (A and B), catalase, and ferritin genes, as potential biomarkers for copper exposure in D. magna regardless of hardness.

Název v anglickém jazyce

Water Hardness Alters the Gene Expression Response and Copper Toxicity in Daphnia magna

Popis výsledku anglicky

The influence of water hardness on copper (Cu) toxicity in Daphnia magna was studied using gene expression analysis. Exposing D. magna to Cu in water with increasing levels of hardness decreased the acute toxicity. Hardness did not affect the predicted Cu complexation. After 24 h, D. magna showed an increased level of genes related to metal homeostasis (mt) following exposure to 25 mu g Cu/L in hard water. Daphnids in soft and medium water responded to 25 mu g Cu/L by upregulation of antioxidant defense and mt genes, revealing oxidative stress as a mechanism of Cu toxicity in D. magna. D. magna exposed to 25 mu g Cu/L in soft water did not survive for 96 h. In contrast, those exposed to 25 mu g Cu/L in medium and hard water survived for 96 h with significantly higher levels of mt genes. The genes related to oxidative damage (heat shock protein and glutathione S-transferase) in these groups did not deviate from control levels, indicating the protective effect of hardness. Metallothionein genes were upregulated at 17 mu g Cu/L at both 24 h and 96 h. The expression of catalase and ferritin increased in this group in soft and hard water at 96 h. The protective effect of hardness (in the tested range) on survival was also observed at a concentration of 25 mu g/L. The results suggest metallothionein (A and B), catalase, and ferritin genes, as potential biomarkers for copper exposure in D. magna regardless of hardness.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fishes

ISSN

2410-3888

e-ISSN

2410-3888

Svazek periodika

7

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

nestrankovano

Kód UT WoS článku

000872724100001

EID výsledku v databázi Scopus

2-s2.0-85140629123