Vliv kryoprotektantů a samců na parametry motility ???

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F06%3A00006946" target="_blank" >RIV/60076658:12610/06:00006946 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Popis výsledku v původním jazyce

A sperm cryopreservation method using different cryoprotectants and sperm from different males was developed. Different percent of pure cryoprotectant (methanol and DMSO) was added in extender 1 or 2 (20 mM tris pH 8 + 30 mM sucrose + 0.5 mM KCl or 20 mMtris pH 8 + 50 mM sucrose + 0.5 mM KCl, dilution 1:1) or non-extended sperm and every 1 ml of mixture was transferred to a 2-ml cryotube. The cryotubes were directly transferred to a pre-programmed PLANER Kryo 10 series III at 0 oC and cooled from 0 oCto -5 oC at a rate of 3 oC.min-1, from -5 oC to -15 oC at a rate of 5 oC.min-1, from -15 oC to -25 oC at a rate of 10 oC .min-1, from -25 oC to -80 oC at a rate of 20 oC .min-1, then held for 5 min at -80 oC and finally transferred into LN2 until next morning. The sperm was thawed in a water bath at 40 oC for 105 s. Fertilization rate of control sperm (unfrozen) samples after 24-h storage at 3 oC was 81.5 % it indicated that the gametes were of good quality. Percentage and the velocity o

Název v anglickém jazyce

Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa

Popis výsledku anglicky

A sperm cryopreservation method using different cryoprotectants and sperm from different males was developed. Different percent of pure cryoprotectant (methanol and DMSO) was added in extender 1 or 2 (20 mM tris pH 8 + 30 mM sucrose + 0.5 mM KCl or 20 mMtris pH 8 + 50 mM sucrose + 0.5 mM KCl, dilution 1:1) or non-extended sperm and every 1 ml of mixture was transferred to a 2-ml cryotube. The cryotubes were directly transferred to a pre-programmed PLANER Kryo 10 series III at 0 oC and cooled from 0 oCto -5 oC at a rate of 3 oC.min-1, from -5 oC to -15 oC at a rate of 5 oC.min-1, from -15 oC to -25 oC at a rate of 10 oC .min-1, from -25 oC to -80 oC at a rate of 20 oC .min-1, then held for 5 min at -80 oC and finally transferred into LN2 until next morning. The sperm was thawed in a water bath at 40 oC for 105 s. Fertilization rate of control sperm (unfrozen) samples after 24-h storage at 3 oC was 81.5 % it indicated that the gametes were of good quality. Percentage and the velocity o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F03%2F0178" target="_blank" >GA524/03/0178: Detailní studium spermií u modelových druhů chrupavčitých (Chondrostei) a kostnatých (Teleostei) ryb</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Ichthyology

ISSN

0175-8659

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

Suppl. 1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

384-388

Kód UT WoS článku

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EID výsledku v databázi Scopus

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