Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation

Popis výsledku v původním jazyce

Gene resources include original Czech fish strains as a part of our national culture heritage (7 strains of common carp and 2 strains of European catfish). In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and European catfish. A sperm freezing procedure was developed, providing good motility of spermatozoa, fertilization and hatching rates in each of the species. Common carp sperm was collected in plastic containers (1/10 - 1/30 volume of sperm andair) and stored under aerobic condition at 4 oC for 1-3 hours. Sperm was diluted 1:5 in a Kurokura extender (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3) and equilibrated for 40 minutes at 4 oC. Diluted sperm was transferred to 2 ml cryotube sand 10 % of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 oC to -9 oC at a rate of 4 oC . min-1, then from -11 oC to -80 oC at a rate of 11 oC .min-1, hold for

Název v anglickém jazyce

Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation

Popis výsledku anglicky

Gene resources include original Czech fish strains as a part of our national culture heritage (7 strains of common carp and 2 strains of European catfish). In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and European catfish. A sperm freezing procedure was developed, providing good motility of spermatozoa, fertilization and hatching rates in each of the species. Common carp sperm was collected in plastic containers (1/10 - 1/30 volume of sperm andair) and stored under aerobic condition at 4 oC for 1-3 hours. Sperm was diluted 1:5 in a Kurokura extender (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3) and equilibrated for 40 minutes at 4 oC. Diluted sperm was transferred to 2 ml cryotube sand 10 % of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 oC to -9 oC at a rate of 4 oC . min-1, then from -11 oC to -80 oC at a rate of 11 oC .min-1, hold for

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

International Conference Joined Meeting of the IIR with the Society for Low Temperature Biology"CRYOPRESERVATION AND SAFE KEEPING OF CELLS AND TISSUES

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

1

Název nakladatele

Society for Low Temperature Biology

Místo vydání

Hradec Králové

Místo konání akce

Hradec Králové

Datum konání akce

13. 5. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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