Imobilizace a kryokonzervace spermatu lína (Tinca tinca L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12610%2F07%3A00007840" target="_blank" >RIV/60076658:12610/07:00007840 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Imobilizing and cryopreservation of tench (Tinca tinca) sperm

Popis výsledku v původním jazyce

In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4°C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4°C to -9°C at a rate of 4°C . min-1, then from -9°C to -80°C at a rate of 11°C . min-1, held for 6 min at -80°C), or a rapid cooling programme L1 (from +4°C to -80°C at a rate of 20°C . min-1, held for 6 min at -80°C) andfinally transferred into liquid N2. The spermatozoa were thwed in water bath 40°C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spe

Název v anglickém jazyce

Imobilizing and cryopreservation of tench (Tinca tinca) sperm

Popis výsledku anglicky

In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4°C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4°C to -9°C at a rate of 4°C . min-1, then from -9°C to -80°C at a rate of 11°C . min-1, held for 6 min at -80°C), or a rapid cooling programme L1 (from +4°C to -80°C at a rate of 20°C . min-1, held for 6 min at -80°C) andfinally transferred into liquid N2. The spermatozoa were thwed in water bath 40°C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spe

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F06%2F0817" target="_blank" >GA524/06/0817: Ultrastruktura, energie a kompetice spermií: Komparativní studie s využitím dvou modelových druhů chrupavčitých a kostnatých ryb</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

272

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

1

Strana od-do

305

Kód UT WoS článku

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EID výsledku v databázi Scopus

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