Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A_____%2F02%3A00004197" target="_blank" >RIV/60076658:_____/02:00004197 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12610/02:00005771

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility

Popis výsledku v původním jazyce

The main aims of the present work were to examine effects of potassium, calcium and immobilizing medium on motility of fresh and demembranated spermatozoa of paddlefish (Polyodon spathula). Evaluation of sperm motility parameters Sperm motility was evaluated for the percentage of motile spermatozoa from video frames, velocity was measured as the distance covered by sperm heads during the corresponding to three video frames and frequency was measured using dark-field microscopy coupled with stroboscopicillumination. Short-term storage of paddlefish sperm in immobilizing medium Seminal plasma of paddlefish has a low spermatozoa concentration (around 0.1-1x109 sperm), therefore it was necessary to concentrate cells by centrifugation (2000 g for 5 min). For examination, 100 #l of centrifuged seminal fluid (transparent part) was diluted with following immobilizing solution (IM): 900#l of seminal fluid, 800 #l of seminal fluid and 100 #l DMSO, 900#l of SM containing 5 mM KCl, 800 #l of SM c

Název v anglickém jazyce

Effects of ions and immobilizing medium on paddlefish (Polyodon spathula) fresh and demembranate sperm motility

Popis výsledku anglicky

The main aims of the present work were to examine effects of potassium, calcium and immobilizing medium on motility of fresh and demembranated spermatozoa of paddlefish (Polyodon spathula). Evaluation of sperm motility parameters Sperm motility was evaluated for the percentage of motile spermatozoa from video frames, velocity was measured as the distance covered by sperm heads during the corresponding to three video frames and frequency was measured using dark-field microscopy coupled with stroboscopicillumination. Short-term storage of paddlefish sperm in immobilizing medium Seminal plasma of paddlefish has a low spermatozoa concentration (around 0.1-1x109 sperm), therefore it was necessary to concentrate cells by centrifugation (2000 g for 5 min). For examination, 100 #l of centrifuged seminal fluid (transparent part) was diluted with following immobilizing solution (IM): 900#l of seminal fluid, 800 #l of seminal fluid and 100 #l DMSO, 900#l of SM containing 5 mM KCl, 800 #l of SM c

Druh

D - Stať ve sborníku

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXth Genetics Days (Proceedings of the Intenational Scientifics Conference

ISBN

80-7157-607-7

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

322-323

Název nakladatele

Mendelova zemědělská a lesnická univerzita

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

12. 9. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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