Použití flow cytometrie pro odhad bakteriálních objemů v přírodních vzorcích planktonu: srovnání s mikroskopickými daty

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F07%3A00087402" target="_blank" >RIV/60077344:_____/07:00087402 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Suitability of flow cytometry for estimating bacterial biovolume in natural plankton samples: comparison with microscopy data

Popis výsledku v původním jazyce

The relationship of flow cytometry data and epifluorescence microscopy measurements was assessed in bacterioplankton samples from 80 lakes to estimate bacterial biovolume and cell size distribution. Total counts of cells (abundances) estimated by both methods were significantly related. To explore the relationship between side scatter (SSC) or DAPI fluorescence data and cell volume (microscopy values), three classes with comparable number of observations were established for each parameter. SSC appearedto be the best cell volume predictor and significant relationships were observed between the frequencies of small classes of both parameters (r2 = 0.545, p< 0.001, n= 80), and in addition between the frequencies of the largest classes (r2 = 0.544, p< 0.001, n= 80). A reliable bacteria biomass prediction can be obtained from SSC frequency classes. FC can be used for a proper estimate of natural bacterioplankton biomass if sample heterogeneity is considered.

Název v anglickém jazyce

Suitability of flow cytometry for estimating bacterial biovolume in natural plankton samples: comparison with microscopy data

Popis výsledku anglicky

The relationship of flow cytometry data and epifluorescence microscopy measurements was assessed in bacterioplankton samples from 80 lakes to estimate bacterial biovolume and cell size distribution. Total counts of cells (abundances) estimated by both methods were significantly related. To explore the relationship between side scatter (SSC) or DAPI fluorescence data and cell volume (microscopy values), three classes with comparable number of observations were established for each parameter. SSC appearedto be the best cell volume predictor and significant relationships were observed between the frequencies of small classes of both parameters (r2 = 0.545, p< 0.001, n= 80), and in addition between the frequencies of the largest classes (r2 = 0.544, p< 0.001, n= 80). A reliable bacteria biomass prediction can be obtained from SSC frequency classes. FC can be used for a proper estimate of natural bacterioplankton biomass if sample heterogeneity is considered.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>R - Projekt Ramcoveho programu EK

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

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Svazek periodika

73

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

4508-4514

Kód UT WoS článku

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EID výsledku v databázi Scopus

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