Regular chondrocyte spacing is a potential cause for coherent ultrasound backscatter in human articular cartilage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21340%2F17%3A00312171" target="_blank" >RIV/68407700:21340/17:00312171 - isvavai.cz</a>

Výsledek na webu

<a href="http://asa.scitation.org/doi/abs/10.1121/1.4979339" target="_blank" >http://asa.scitation.org/doi/abs/10.1121/1.4979339</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1121/1.4979339" target="_blank" >10.1121/1.4979339</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Regular chondrocyte spacing is a potential cause for coherent ultrasound backscatter in human articular cartilage

Popis výsledku v původním jazyce

The potential of quantitative ultrasound (QUS) to assess the regular cellular spacing in the superficial cartilage zones was investigated experimentally and numerically. Nine osteochondral samples, extracted from two human cadaver knee joints, were measured using a 50-MHz ultrasound scanning device and evaluated using Mankin score. Simulated backscattered power spectra from models with an idealized cell alignment exhibited a pronounced frequency peak. From the peak, cell spacing in the range between 15 and 40 μm between cell layers was detected with an average error of 0.2 μm. The mean QUS-based cell spacing was 28.3 ± 5.3 μm. Strong correlation (R2 = 0.59, p <= 0.001) between spacing estimates from light microscopy (LM) and QUS was found for samples with Mankin score <=3. For higher scores, QUS-based spacing was significantly higher (p <= 0.05) compared to LM-based spacing. QUS-based spacing estimates together with other QUS parameters may serve as future biomarkers for detecting early signs of osteoarthrosis.

Název v anglickém jazyce

Regular chondrocyte spacing is a potential cause for coherent ultrasound backscatter in human articular cartilage

Popis výsledku anglicky

The potential of quantitative ultrasound (QUS) to assess the regular cellular spacing in the superficial cartilage zones was investigated experimentally and numerically. Nine osteochondral samples, extracted from two human cadaver knee joints, were measured using a 50-MHz ultrasound scanning device and evaluated using Mankin score. Simulated backscattered power spectra from models with an idealized cell alignment exhibited a pronounced frequency peak. From the peak, cell spacing in the range between 15 and 40 μm between cell layers was detected with an average error of 0.2 μm. The mean QUS-based cell spacing was 28.3 ± 5.3 μm. Strong correlation (R2 = 0.59, p <= 0.001) between spacing estimates from light microscopy (LM) and QUS was found for samples with Mankin score <=3. For higher scores, QUS-based spacing was significantly higher (p <= 0.05) compared to LM-based spacing. QUS-based spacing estimates together with other QUS parameters may serve as future biomarkers for detecting early signs of osteoarthrosis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10307 - Acoustics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of the Acoustical Society of America

ISSN

0001-4966

e-ISSN

1520-8524

Svazek periodika

141

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

3105-3116

Kód UT WoS článku

000403270600026

EID výsledku v databázi Scopus

2-s2.0-85019043455