Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381545" target="_blank" >RIV/60077344:_____/12:00381545 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883615

Výsledek na webu

<a href="http://dx.doi.org/10.1515/hsz-2011-0286" target="_blank" >http://dx.doi.org/10.1515/hsz-2011-0286</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/hsz-2011-0286" target="_blank" >10.1515/hsz-2011-0286</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla)

Popis výsledku v původním jazyce

Viroid-caused pathogenesis is a speci?c process dependent on viroid and host genotype(s), and may involve viroid-speci?c small RNAs (vsRNA). We describe a new PSTVd variant C3, evolved through sequence adaptation to the host chamomile, causing extraordinary severe symptoms. The symptoms and the vsRNA pool of PSTVd-C3 were compared to those of severe variant PSTVd-AS1, from which PSTVd-C3 differs by 5 mutations within the P domain. PSTVd-C3 and -AS1 caused similar symptoms on chamomile, tomato, and Nicotiana benthamiana, including a block of lignin biosynthesis in roots of cultured chamomile and tomato. These strains exhibited similar but species-speci?c distributions of selected vsRNAs, as quanti?ed using TaqMan probes, with higher stability and lowervariation in analyzed vsRNA pools than the mild PSTVd-QFA. Strong downregulation of four selected expression markers? (TCP3, CIPK, VSF-1, and VPE) was detected in tomato leaf blades infected by PSTVd-C3- and -AS1 but not by PSTVd-QFA.

Název v anglickém jazyce

Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla)

Popis výsledku anglicky

Viroid-caused pathogenesis is a speci?c process dependent on viroid and host genotype(s), and may involve viroid-speci?c small RNAs (vsRNA). We describe a new PSTVd variant C3, evolved through sequence adaptation to the host chamomile, causing extraordinary severe symptoms. The symptoms and the vsRNA pool of PSTVd-C3 were compared to those of severe variant PSTVd-AS1, from which PSTVd-C3 differs by 5 mutations within the P domain. PSTVd-C3 and -AS1 caused similar symptoms on chamomile, tomato, and Nicotiana benthamiana, including a block of lignin biosynthesis in roots of cultured chamomile and tomato. These strains exhibited similar but species-speci?c distributions of selected vsRNAs, as quanti?ed using TaqMan probes, with higher stability and lowervariation in analyzed vsRNA pools than the mild PSTVd-QFA. Strong downregulation of four selected expression markers? (TCP3, CIPK, VSF-1, and VPE) was detected in tomato leaf blades infected by PSTVd-C3- and -AS1 but not by PSTVd-QFA.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GCP501%2F10%2FJ018" target="_blank" >GCP501/10/J018: Pospiviroidní patogeneze jako regulační porucha zprostředkovaná malými viroid-specifickými RNA.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biological Chemistry

ISSN

1431-6730

e-ISSN

—

Svazek periodika

393

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

605-615

Kód UT WoS článku

000307087600007

EID výsledku v databázi Scopus

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