Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381618" target="_blank" >RIV/60077344:_____/12:00381618 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883364

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gks333" target="_blank" >http://dx.doi.org/10.1093/nar/gks333</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks333" target="_blank" >10.1093/nar/gks333</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET

Popis výsledku v původním jazyce

Here we explored parameters critical for characterization of NA structure using FRET in the complex cellular environment of a living E. coli cell. Measurements indicated that the uncertainty in determining the orientation factor of fluorophores, which translates into precision of inter-probe distance determination, was the main limiting factor for the quantitative structural analysis of nucleic acids in living cells. Our data suggest that despite its low precision, the in-cell FRET data could be appliedto assess the consistency of structural data acquired from FRET efficiency values measured in a complex cellular environment with various structural models. The main advantage of the in-cell spFRET setup presented here over other established techniquesallowing conformational analysis in vivo is that it allows structural investigation of NA structure in any cell type and, most importantly, in a native cellular environment, which is not disturbed by either introduced bulk NA or by the us

Název v anglickém jazyce

Towards characterization of DNA structure under physiological conditions in vivo at the single-molecule level using single-pair FRET

Popis výsledku anglicky

Here we explored parameters critical for characterization of NA structure using FRET in the complex cellular environment of a living E. coli cell. Measurements indicated that the uncertainty in determining the orientation factor of fluorophores, which translates into precision of inter-probe distance determination, was the main limiting factor for the quantitative structural analysis of nucleic acids in living cells. Our data suggest that despite its low precision, the in-cell FRET data could be appliedto assess the consistency of structural data acquired from FRET efficiency values measured in a complex cellular environment with various structural models. The main advantage of the in-cell spFRET setup presented here over other established techniquesallowing conformational analysis in vivo is that it allows structural investigation of NA structure in any cell type and, most importantly, in a native cellular environment, which is not disturbed by either introduced bulk NA or by the us

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/KAN200100801" target="_blank" >KAN200100801: Bioaktivní biokompatibilní povrchy a nové nanostrukturované kompozity pro aplikace v medicíně a farmacii</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000308959800002

EID výsledku v databázi Scopus

—