Imaging of tissue sections with very slow electrons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00436878" target="_blank" >RIV/60077344:_____/15:00436878 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/15:00436878

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ultramic.2014.10.009" target="_blank" >http://dx.doi.org/10.1016/j.ultramic.2014.10.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ultramic.2014.10.009" target="_blank" >10.1016/j.ultramic.2014.10.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Imaging of tissue sections with very slow electrons

Popis výsledku v původním jazyce

The examination of thin sections of tissues with electron microscopes is an indispensable tool. Being composed of light elements, samples of living matter illuminated with electrons at the usual high energies of tens or even hundreds of kiloelectronvoltsprovide very low image contrasts in transmission or scanning transmission electron microscopes. Therefore, heavy metal salts are added to the specimen during preparation procedures (post-fixation with osmium tetroxide or staining). However, these procedures can modify or obscure the ultrastructural details of cells. Here we show that the energy of electrons used for the scanned transmission imaging of tissue sections can be reduced to mere hundreds or even tens of electronvolts and can produce extremely high contrast even for samples free of any metal salts. We found that when biasing a sufficiently thin tissue section sample to a high negative potential in a scanning transmission electron microscope, thereby reducing the energy of the

Název v anglickém jazyce

Imaging of tissue sections with very slow electrons

Popis výsledku anglicky

The examination of thin sections of tissues with electron microscopes is an indispensable tool. Being composed of light elements, samples of living matter illuminated with electrons at the usual high energies of tens or even hundreds of kiloelectronvoltsprovide very low image contrasts in transmission or scanning transmission electron microscopes. Therefore, heavy metal salts are added to the specimen during preparation procedures (post-fixation with osmium tetroxide or staining). However, these procedures can modify or obscure the ultrastructural details of cells. Here we show that the energy of electrons used for the scanned transmission imaging of tissue sections can be reduced to mere hundreds or even tens of electronvolts and can produce extremely high contrast even for samples free of any metal salts. We found that when biasing a sufficiently thin tissue section sample to a high negative potential in a scanning transmission electron microscope, thereby reducing the energy of the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Ultramicroscopy

ISSN

0304-3991

e-ISSN

—

Svazek periodika

148

Číslo periodika v rámci svazku

JAN 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

146-150

Kód UT WoS článku

000345973000018

EID výsledku v databázi Scopus

2-s2.0-84909592404