Limited effect of adaptive immune response to control encephalitozoonosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00483936" target="_blank" >RIV/60077344:_____/17:00483936 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/17:43896924

Výsledek na webu

<a href="http://dx.doi.org/10.1111/pim.12496" target="_blank" >http://dx.doi.org/10.1111/pim.12496</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pim.12496" target="_blank" >10.1111/pim.12496</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Limited effect of adaptive immune response to control encephalitozoonosis

Popis výsledku v původním jazyce

This study revises our understanding of the effectiveness of cell-mediated adaptive immunity and treatment against microsporidia using molecular detection and quantification of microsporidia in immunocompetent C57Bl/6 and immunodeficient CD4(-/-) and CD8(-/-) mice for the first time. We demonstrate an intense dissemination of microsporidia into most organs within the first weeks post-infection in all strains of mice, followed by a chronic infection characterized by microsporidia persistence in CD4(-/-) and C57Bl/6 mice and a lethal outcome for CD8(-/-) mice. Albendazole application reduces microsporidia burden in C57Bl/6 and CD4(-/-) mice, whereas CD8(-/-) mice experience only a temporary effect of the treatment. Surprisingly, treated CD8(-/-) mice survived the entire experimental duration despite enormous microsporidia burden. On the basis of our results, we conclude that microsporidia survive despite the presence of immune mechanisms and treatments that are currently considered to be effective and therefore that CD8 T lymphocytes represent a major, but not sole effector mechanism controlling microsporidiosis. Furthermore, the survival of mice does not correspond to spore burden, which provides new insight into latent microsporidiosis from an epidemiological point of view.

Název v anglickém jazyce

Limited effect of adaptive immune response to control encephalitozoonosis

Popis výsledku anglicky

This study revises our understanding of the effectiveness of cell-mediated adaptive immunity and treatment against microsporidia using molecular detection and quantification of microsporidia in immunocompetent C57Bl/6 and immunodeficient CD4(-/-) and CD8(-/-) mice for the first time. We demonstrate an intense dissemination of microsporidia into most organs within the first weeks post-infection in all strains of mice, followed by a chronic infection characterized by microsporidia persistence in CD4(-/-) and C57Bl/6 mice and a lethal outcome for CD8(-/-) mice. Albendazole application reduces microsporidia burden in C57Bl/6 and CD4(-/-) mice, whereas CD8(-/-) mice experience only a temporary effect of the treatment. Surprisingly, treated CD8(-/-) mice survived the entire experimental duration despite enormous microsporidia burden. On the basis of our results, we conclude that microsporidia survive despite the presence of immune mechanisms and treatments that are currently considered to be effective and therefore that CD8 T lymphocytes represent a major, but not sole effector mechanism controlling microsporidiosis. Furthermore, the survival of mice does not correspond to spore burden, which provides new insight into latent microsporidiosis from an epidemiological point of view.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasite immunology

ISSN

0141-9838

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000416233100002

EID výsledku v databázi Scopus

2-s2.0-85035033255