Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00554391" target="_blank" >RIV/60077344:_____/21:00554391 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.pnas.org/content/118/45/e2109381118" target="_blank" >https://www.pnas.org/content/118/45/e2109381118</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.2109381118" target="_blank" >10.1073/pnas.2109381118</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness

Popis výsledku v původním jazyce

Methyl farnesoate (MF) plays hormonal regulatory roles in crustaceans. An epoxidated form of MF, known as juvenile hormone (JH), controls metamorphosis and stimulates reproduction in insects. To address the evolutionary significance of MF epoxidation, we generated mosquitoes completely lacking either of the two enzymes that catalyze the last steps of MF/JH biosynthesis and epoxidation, respectively: the JH acid methyltransferase (JHAMT) and the P450 epoxidase CYP15 (EPDX). jhamt(-/-) larvae lacking both MF and JH died at the onset of metamorphosis. Strikingly, epox(-/-) mutants, which synthesized MF but no 1H, completed the entire life cycle. While epox(-/-) adults were fertile, the reproductive performance of both sexes was dramatically reduced. Our results suggest that although MF can substitute for the absence of JH in mosquitoes, it is with a significant fitness cost. We propose that MF can fulfill most roles of JH, but its epoxidation to JH was a key innovation providing insects with a reproductive advantage.

Název v anglickém jazyce

Epoxidation of juvenile hormone was a key innovation improving insect reproductive fitness

Popis výsledku anglicky

Methyl farnesoate (MF) plays hormonal regulatory roles in crustaceans. An epoxidated form of MF, known as juvenile hormone (JH), controls metamorphosis and stimulates reproduction in insects. To address the evolutionary significance of MF epoxidation, we generated mosquitoes completely lacking either of the two enzymes that catalyze the last steps of MF/JH biosynthesis and epoxidation, respectively: the JH acid methyltransferase (JHAMT) and the P450 epoxidase CYP15 (EPDX). jhamt(-/-) larvae lacking both MF and JH died at the onset of metamorphosis. Strikingly, epox(-/-) mutants, which synthesized MF but no 1H, completed the entire life cycle. While epox(-/-) adults were fertile, the reproductive performance of both sexes was dramatically reduced. Our results suggest that although MF can substitute for the absence of JH in mosquitoes, it is with a significant fitness cost. We propose that MF can fulfill most roles of JH, but its epoxidation to JH was a key innovation providing insects with a reproductive advantage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

<a href="/cs/project/GX20-05151X" target="_blank" >GX20-05151X: Nalezení chybějících genetických komponent a chemických regulátorů signalizace juvenilního hormonu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

e-ISSN

—

Svazek periodika

118

Číslo periodika v rámci svazku

45

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

e2109381118

Kód UT WoS článku

000720928400015

EID výsledku v databázi Scopus

2-s2.0-85119276601