Melittin—The principal toxin of honeybee venom—Is also produced in the honeybee fat body

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00585498" target="_blank" >RIV/60077344:_____/24:00585498 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/24:43907982 RIV/60076658:12520/24:43907982

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1532045624000966/pdfft?md5=3664f989524bf5def364284a6ad5b78b&pid=1-s2.0-S1532045624000966-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1532045624000966/pdfft?md5=3664f989524bf5def364284a6ad5b78b&pid=1-s2.0-S1532045624000966-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpc.2024.109928" target="_blank" >10.1016/j.cbpc.2024.109928</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Melittin—The principal toxin of honeybee venom—Is also produced in the honeybee fat body

Popis výsledku v původním jazyce

Melittin is a powerful toxin present in honeybee venom that is active in a wide range of animals, from insects to humans. Melittin exerts numerous biological, toxicological, and pharmacological effects, the most important of which is destruction of the cell membrane. The phospholipase activity of melittin and its ability to activate phospholipases in the venom contribute to these actions. Using analytical methods, we discovered that the honeybee Apis mellifera produces melittin not only in the venom gland but also in its fat body cells, which remain resistant to this toxin's effects. We suggest that melittin acts as an anti-bacterial agent, since its gene expression is significantly upregulated when honeybees are infected with Escherichia coli and Listeria monocytogenes bacteria, additionally, melittin effectively kills these bacteria in the disc diffusion test. We hypothesize that the chemical and physicochemical properties of the melittin molecule (hydrophilicity, lipophilicity, and capacity to form tetramers) in combination with reactive conditions (melittin concentration, salt concentration, pH, and temperature) are responsible for the targeted destruction of bacterial cells and apparent tolerance towards own tissue cells. Considering that melittin is an important current and, importantly, potential broad-spectrum medication, a thorough understanding of the observed phenomena may significantly increase its use in clinical practice.

Název v anglickém jazyce

Melittin—The principal toxin of honeybee venom—Is also produced in the honeybee fat body

Popis výsledku anglicky

Melittin is a powerful toxin present in honeybee venom that is active in a wide range of animals, from insects to humans. Melittin exerts numerous biological, toxicological, and pharmacological effects, the most important of which is destruction of the cell membrane. The phospholipase activity of melittin and its ability to activate phospholipases in the venom contribute to these actions. Using analytical methods, we discovered that the honeybee Apis mellifera produces melittin not only in the venom gland but also in its fat body cells, which remain resistant to this toxin's effects. We suggest that melittin acts as an anti-bacterial agent, since its gene expression is significantly upregulated when honeybees are infected with Escherichia coli and Listeria monocytogenes bacteria, additionally, melittin effectively kills these bacteria in the disc diffusion test. We hypothesize that the chemical and physicochemical properties of the melittin molecule (hydrophilicity, lipophilicity, and capacity to form tetramers) in combination with reactive conditions (melittin concentration, salt concentration, pH, and temperature) are responsible for the targeted destruction of bacterial cells and apparent tolerance towards own tissue cells. Considering that melittin is an important current and, importantly, potential broad-spectrum medication, a thorough understanding of the observed phenomena may significantly increase its use in clinical practice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA24-10662S" target="_blank" >GA24-10662S: Obranné reakce proti jedu vosičky Habrobracon hebetor a včely medonosné: mechanismus působení</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Comparative Biochemistry and Physiology Part C

ISSN

1532-0456

e-ISSN

1878-1659

Svazek periodika

281

Číslo periodika v rámci svazku

JUL 01

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

109928

Kód UT WoS článku

001218562100002

EID výsledku v databázi Scopus

2-s2.0-85190996531