Molecular Characterization of a Novel Rubodvirus Infecting Raspberries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604533" target="_blank" >RIV/60077344:_____/24:00604533 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/24:43908504 RIV/25271121:_____/24:N0000126

Výsledek na webu

<a href="https://www.mdpi.com/1999-4915/16/7/1074" target="_blank" >https://www.mdpi.com/1999-4915/16/7/1074</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v16071074" target="_blank" >10.3390/v16071074</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Characterization of a Novel Rubodvirus Infecting Raspberries

Popis výsledku v původním jazyce

A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants in the Czech Republic and has tentatively been named raspberry rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within the group, albeit distantly related to other members. A screening of 679 plant and 168 arthropod samples from the Czech Republic and Norway revealed RaRV1 in 10 raspberry shrubs, one batch of Aphis idaei, and one individual of Orius minutus. Furthermore, a distinct isolate of this virus was found, sharing 95% amino acid identity in both the full nucleoprotein and partial sequence of the RNA-dependent RNA polymerase gene sequences, meeting the species demarcation criteria. This discovery marks the first reported instance of a rubodvirus infecting raspberry plants. Although transmission experiments under experimental conditions were unsuccessful, positive detection of the virus in some insects suggests their potential role as vectors for the virus.

Název v anglickém jazyce

Molecular Characterization of a Novel Rubodvirus Infecting Raspberries

Popis výsledku anglicky

A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants in the Czech Republic and has tentatively been named raspberry rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within the group, albeit distantly related to other members. A screening of 679 plant and 168 arthropod samples from the Czech Republic and Norway revealed RaRV1 in 10 raspberry shrubs, one batch of Aphis idaei, and one individual of Orius minutus. Furthermore, a distinct isolate of this virus was found, sharing 95% amino acid identity in both the full nucleoprotein and partial sequence of the RNA-dependent RNA polymerase gene sequences, meeting the species demarcation criteria. This discovery marks the first reported instance of a rubodvirus infecting raspberry plants. Although transmission experiments under experimental conditions were unsuccessful, positive detection of the virus in some insects suggests their potential role as vectors for the virus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses

ISSN

1999-4915

e-ISSN

1999-4915

Svazek periodika

16

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

16

Strana od-do

1074

Kód UT WoS článku

001277695300001

EID výsledku v databázi Scopus

2-s2.0-85199672012