Comprehensive tools for culturing Blastocystis: A standardized resource for research and diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00638364" target="_blank" >RIV/60077344:_____/25:00638364 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/cpz1.70175" target="_blank" >https://doi.org/10.1002/cpz1.70175</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cpz1.70175" target="_blank" >10.1002/cpz1.70175</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comprehensive tools for culturing Blastocystis: A standardized resource for research and diagnostics

Popis výsledku v původním jazyce

Blastocystis spp. is a widely prevalent anaerobic protozoan of uncertain pathogenicity found in the gastrointestinal tracts of over 1 billion people worldwide. Despite its potential significance in health and disease, Blastocystis spp. remains challenging to culture axenically due to its anaerobic nature and the diversity of its genetic subtypes. This manuscript presents a standardized toolkit for culturing Blastocystis spp. in xenic and axenic conditions, detailing protocols for the preparation of appropriate liquid and solid media, cryopreservation, and inoculation. By providing a comprehensive set of tools and methodologies, this work aims to streamline research on Blastocystis spp., enabling reproducibility, subtype characterization, and advancements in understanding its role in the gut microbiome and host health. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Establishment of xenic Blastocystis culture from stool samples in liquid mediumBasic Protocol 2: Subculturing from existing Blastocystis liquid cultureSupport Protocol 1: Preparation of modified Jones' medium for Blastocystis culturingSupport Protocol 2: Preparation of trypticase-yeast extract-serum-gastric mucin-9 (TSGYM-9) medium for Blastocystis culturingSupport Protocol 3: Preparation of liver extract-yeast extract-serum-gastric mucin (LYSGM) medium for Blastocystis culturingBasic Protocol 3: Subculturing xenic Blastocystis cultures in diphasic mediumSupport Protocol 4: Preparation of Robinson's medium for Blastocystis culturingSupport Protocol 5: Preparation of Ringer's agar slants for Blastocystis culturingBasic Protocol 4: Axenization of Blastocystis culturesBasic Protocol 5: Subculturing axenic Blastocystis cultures in diphasic mediumSupport Protocol 6: Preparation of Boeck and Drbohlav's Locke-egg serum (LES) medium for Blastocystis culturingSupport Protocol 7: Preparation of Boeck and Drbohlav's diphasic modified medium (BDMM) for Blastocystis culturingBasic Protocol 6: Establishment of axenic Blastocystis cultures in Iscove's modified Dulbecco's medium (IMDM)Basic Protocol 7: Establishment of axenic Blastocystis cultures in soft IMDM agarBasic Protocol 8: Establishment of axenic Blastocystis cultures on solid IMDM agarBasic Protocol 9: Optimized method for establishing axenic Blastocystis cultures on solid IMDM agarBasic Protocol 10: Establishment of axenic Blastocystis cultures in semi-solid Locke's agarBasic Protocol 11: Cryopreservation of xenic Blastocystis culturesBasic Protocol 12: Cryopreservation of axenic Blastocystis culturesBasic Protocol 13: Inoculation of liquid medium with xenic Blastocystis cultures from frozen stocksBasic Protocol 14: Inoculation of liquid medium with axenic Blastocystis cultures from frozen stocks

Název v anglickém jazyce

Comprehensive tools for culturing Blastocystis: A standardized resource for research and diagnostics

Popis výsledku anglicky

Blastocystis spp. is a widely prevalent anaerobic protozoan of uncertain pathogenicity found in the gastrointestinal tracts of over 1 billion people worldwide. Despite its potential significance in health and disease, Blastocystis spp. remains challenging to culture axenically due to its anaerobic nature and the diversity of its genetic subtypes. This manuscript presents a standardized toolkit for culturing Blastocystis spp. in xenic and axenic conditions, detailing protocols for the preparation of appropriate liquid and solid media, cryopreservation, and inoculation. By providing a comprehensive set of tools and methodologies, this work aims to streamline research on Blastocystis spp., enabling reproducibility, subtype characterization, and advancements in understanding its role in the gut microbiome and host health. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Establishment of xenic Blastocystis culture from stool samples in liquid mediumBasic Protocol 2: Subculturing from existing Blastocystis liquid cultureSupport Protocol 1: Preparation of modified Jones' medium for Blastocystis culturingSupport Protocol 2: Preparation of trypticase-yeast extract-serum-gastric mucin-9 (TSGYM-9) medium for Blastocystis culturingSupport Protocol 3: Preparation of liver extract-yeast extract-serum-gastric mucin (LYSGM) medium for Blastocystis culturingBasic Protocol 3: Subculturing xenic Blastocystis cultures in diphasic mediumSupport Protocol 4: Preparation of Robinson's medium for Blastocystis culturingSupport Protocol 5: Preparation of Ringer's agar slants for Blastocystis culturingBasic Protocol 4: Axenization of Blastocystis culturesBasic Protocol 5: Subculturing axenic Blastocystis cultures in diphasic mediumSupport Protocol 6: Preparation of Boeck and Drbohlav's Locke-egg serum (LES) medium for Blastocystis culturingSupport Protocol 7: Preparation of Boeck and Drbohlav's diphasic modified medium (BDMM) for Blastocystis culturingBasic Protocol 6: Establishment of axenic Blastocystis cultures in Iscove's modified Dulbecco's medium (IMDM)Basic Protocol 7: Establishment of axenic Blastocystis cultures in soft IMDM agarBasic Protocol 8: Establishment of axenic Blastocystis cultures on solid IMDM agarBasic Protocol 9: Optimized method for establishing axenic Blastocystis cultures on solid IMDM agarBasic Protocol 10: Establishment of axenic Blastocystis cultures in semi-solid Locke's agarBasic Protocol 11: Cryopreservation of xenic Blastocystis culturesBasic Protocol 12: Cryopreservation of axenic Blastocystis culturesBasic Protocol 13: Inoculation of liquid medium with xenic Blastocystis cultures from frozen stocksBasic Protocol 14: Inoculation of liquid medium with axenic Blastocystis cultures from frozen stocks

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Protocols

ISSN

2691-1299

e-ISSN

2691-1299

Svazek periodika

5

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

36

Strana od-do

e70175

Kód UT WoS článku

001550500900001

EID výsledku v databázi Scopus

2-s2.0-105013536601