Simple and rapid procedure for at the determination of individual free fatty acids in serum.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077395%3A_____%2F02%3A60023024" target="_blank" >RIV/60077395:_____/02:60023024 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simple and rapid procedure for at the determination of individual free fatty acids in serum.

Popis výsledku v původním jazyce

A microscale procedure for a rapid extraction, re-extraction and methylation of serum or plasma free fatty acids (FFAs) is described. There is no necessity to evaporate extracting solvent or heat the reaction medium to prepare corresponding methyl estersfor gas chromatographic analysis. The approach comprises three consecutive steps, carried out in two reaction vials, during which the neutral lipids are removed and the free fatty acids re-extracted and subsequently methylated. Methylation succeds uponusing methyl chloroformate in combination with a non-smelling organic base within seconds. The method proved to be highly reproducible, with intraassay variation coefficients being less than 5 % for the major serum fatty acids.

Název v anglickém jazyce

Simple and rapid procedure for at the determination of individual free fatty acids in serum.

Popis výsledku anglicky

A microscale procedure for a rapid extraction, re-extraction and methylation of serum or plasma free fatty acids (FFAs) is described. There is no necessity to evaporate extracting solvent or heat the reaction medium to prepare corresponding methyl estersfor gas chromatographic analysis. The approach comprises three consecutive steps, carried out in two reaction vials, during which the neutral lipids are removed and the free fatty acids re-extracted and subsequently methylated. Methylation succeds uponusing methyl chloroformate in combination with a non-smelling organic base within seconds. The method proved to be highly reproducible, with intraassay variation coefficients being less than 5 % for the major serum fatty acids.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

465

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

433-439

Kód UT WoS článku

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EID výsledku v databázi Scopus

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