Use of cryopreservation for long-term storage of potato germplasm in the Czech Republic

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of cryopreservation for long-term storage of potato germplasm in the Czech Republic

Popis výsledku v původním jazyce

Long-term storage of potato germplasm is usually carried out by means of in vitro cultivation of potato explants at slow-growth condition or by cryopreservation methods. In the Czech Republic the complete germplasm collection is kept at the Potato Research Institute in Havlíčkův Brod under slow-growth conditions in in vitro conditions. To improve standards of germplasm storage a safety backup for the most valuable accessions was established at the Czech Cryobank of Plants and Microorganisms in the Crop Research Institute in Prague. The advantage of the slow-growth conditions is availability of explants for their multiplication and subsequent testing or distribution. Potato seeds are produced in shorter time when compared with cryopreservation. The disadvantage of this method is a risk of callus formation connected with somaclonal variation, and potential microorganism contamination. In contrast, cryopreservation at ultra-low temperatures is more secure against contamination by microorganisms. At the same time, plant growth and development are completely stopped; biochemical and genetic changes are excluded due to vitrification status of plant material at ultra-low temperature. Thus, the cryopreservation method is preferable for long-term storage of potato germplasm.

Název v anglickém jazyce

Use of cryopreservation for long-term storage of potato germplasm in the Czech Republic

Popis výsledku anglicky

Long-term storage of potato germplasm is usually carried out by means of in vitro cultivation of potato explants at slow-growth condition or by cryopreservation methods. In the Czech Republic the complete germplasm collection is kept at the Potato Research Institute in Havlíčkův Brod under slow-growth conditions in in vitro conditions. To improve standards of germplasm storage a safety backup for the most valuable accessions was established at the Czech Cryobank of Plants and Microorganisms in the Crop Research Institute in Prague. The advantage of the slow-growth conditions is availability of explants for their multiplication and subsequent testing or distribution. Potato seeds are produced in shorter time when compared with cryopreservation. The disadvantage of this method is a risk of callus formation connected with somaclonal variation, and potential microorganism contamination. In contrast, cryopreservation at ultra-low temperatures is more secure against contamination by microorganisms. At the same time, plant growth and development are completely stopped; biochemical and genetic changes are excluded due to vitrification status of plant material at ultra-low temperature. Thus, the cryopreservation method is preferable for long-term storage of potato germplasm.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40101 - Agriculture

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů