Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875219" target="_blank" >RIV/60162694:G44__/14:43875219 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.nel.edu/archive_issues/o/35_s2/35_s2_Pohanka_155-157.pdf" target="_blank" >http://www.nel.edu/archive_issues/o/35_s2/35_s2_Pohanka_155-157.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model

Popis výsledku v původním jazyce

OBJECTIVES: HI-6 or asoxime in some sources is an antidotum for nerve agents. In recent experiments, implication of HI-6 in immunity response was proved; however, the issue was not studied in details. In this experiment, role of cytokines in HI-6 impacton immunity was searched. DESIGN: BALB/c mice were exposed to saline, HI-6 in a dose 1 - 100 mg/kg and/or 1 keyhole limpet hemocyanin (KLH) 1 mg/kg. Mice were sacrificed 21 days after experiment beginning and interleukins (IL) 1, 2, 4, 6 were determinedby Enzyme Linked Immunosorbent Assay (ELISA). RESULTS: The animals had no pathological manifestation. From the tested cytokines, no significant alteration was found for the IL-1, IL-4 and IL-6. IL-2 was significantly increased in a dose response manner.CONCLUSIONS: The experimental data well correlates with the previous work where HI-6 caused increase of antibodies production. HI-6 is suitable to be used as an adjuvant whenever immunity should be pharmacologically altered.

Název v anglickém jazyce

Effect of HI-6 on cytokines production after immunity stimulation by keyhole limpet hemocyanin in a mouse model

Popis výsledku anglicky

OBJECTIVES: HI-6 or asoxime in some sources is an antidotum for nerve agents. In recent experiments, implication of HI-6 in immunity response was proved; however, the issue was not studied in details. In this experiment, role of cytokines in HI-6 impacton immunity was searched. DESIGN: BALB/c mice were exposed to saline, HI-6 in a dose 1 - 100 mg/kg and/or 1 keyhole limpet hemocyanin (KLH) 1 mg/kg. Mice were sacrificed 21 days after experiment beginning and interleukins (IL) 1, 2, 4, 6 were determinedby Enzyme Linked Immunosorbent Assay (ELISA). RESULTS: The animals had no pathological manifestation. From the tested cytokines, no significant alteration was found for the IL-1, IL-4 and IL-6. IL-2 was significantly increased in a dose response manner.CONCLUSIONS: The experimental data well correlates with the previous work where HI-6 caused increase of antibodies production. HI-6 is suitable to be used as an adjuvant whenever immunity should be pharmacologically altered.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LH11023" target="_blank" >LH11023: Vylepšení vakcinační účinnosti pomocí cholinergní protizánětlivé dráhy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neuroendocrinology Letters

ISSN

0172-780X

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

Suppl 2

Stát vydavatele periodika

SE - Švédské království

Počet stran výsledku

3

Strana od-do

155-157

Kód UT WoS článku

000351064900020

EID výsledku v databázi Scopus

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