Colorimetric sol gel based biosensor platform for determination of reduced glutathione
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875670" target="_blank" >RIV/60162694:G44__/16:43875670 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43410/16:43910864
Výsledek na webu
<a href="http://www.sciencedirect.com/science/article/pii/S0925400516308644" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0925400516308644</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2016.06.012" target="_blank" >10.1016/j.snb.2016.06.012</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Colorimetric sol gel based biosensor platform for determination of reduced glutathione
Popis výsledku v původním jazyce
Reduced glutathione (GSH) is a biochemical and antioxidant marker in various examinations of pathological states. Though standard tests for determination GSH in biological samples are available, there are significant drawbacks. The method for determination of GSH based on enzymatic reaction applying enzyme glutathione-S-transferase (GST) was established and introduced to provide a simple but reliable platform for determination of GSH in biological samples. Sol-gel membrane was utilized for immobilization of the homogenate from pig liver containing high activity of GST. Enzymatic method was compared with currently used Ellman method. The results of experiments showed, that Ellman method as well as enzymatic sol-gel method are appropriate for the determination of GSH in tissues. Michaelis-Menten constant of the novel method was set to be 1.9 mmol/l and limit of detection (LOD) of Ellman method was determined to be 30 mu mol/l while LOD of enzymatic method was set to be six times higher. Concerning to interferences, enzymatic sol-gel technique showed better results compared to the standard Ellman method. No interfering substances were revealed in the GST based assay. On the other hand, bovine serum albumin, ascorbic acid (AA) and N-acetylcysteine (N-AC) were confirmed as strong interferents in Ellman method. Spiked plasma samples were measured using the GST based method and their kinetic curve has very similar shape as kinetic curve of standard solutions.
Název v anglickém jazyce
Colorimetric sol gel based biosensor platform for determination of reduced glutathione
Popis výsledku anglicky
Reduced glutathione (GSH) is a biochemical and antioxidant marker in various examinations of pathological states. Though standard tests for determination GSH in biological samples are available, there are significant drawbacks. The method for determination of GSH based on enzymatic reaction applying enzyme glutathione-S-transferase (GST) was established and introduced to provide a simple but reliable platform for determination of GSH in biological samples. Sol-gel membrane was utilized for immobilization of the homogenate from pig liver containing high activity of GST. Enzymatic method was compared with currently used Ellman method. The results of experiments showed, that Ellman method as well as enzymatic sol-gel method are appropriate for the determination of GSH in tissues. Michaelis-Menten constant of the novel method was set to be 1.9 mmol/l and limit of detection (LOD) of Ellman method was determined to be 30 mu mol/l while LOD of enzymatic method was set to be six times higher. Concerning to interferences, enzymatic sol-gel technique showed better results compared to the standard Ellman method. No interfering substances were revealed in the GST based assay. On the other hand, bovine serum albumin, ascorbic acid (AA) and N-acetylcysteine (N-AC) were confirmed as strong interferents in Ellman method. Spiked plasma samples were measured using the GST based method and their kinetic curve has very similar shape as kinetic curve of standard solutions.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Sensors and Actuators B-Chemical
ISSN
0925-4005
e-ISSN
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Svazek periodika
236
Číslo periodika v rámci svazku
June
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
8
Strana od-do
442-449
Kód UT WoS článku
000382229700053
EID výsledku v databázi Scopus
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