Construction of a QCM Biosensor for free Hemoglobin Assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00536959" target="_blank" >RIV/60162694:G44__/19:00536959 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol14/140605237.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol14/140605237.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2019.06.48" target="_blank" >10.20964/2019.06.48</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Construction of a QCM Biosensor for free Hemoglobin Assay

Popis výsledku v původním jazyce

Protein responsible for oxygen transfer, hemoglobin, is an important marker in biochemistry that can be measured in blood and blood plasma. Meaning of the assays are different in the two fluids. While blood hemoglobin serving as a marker of anemia and some oncotic processes is simply measurable by spectroscopy, hemoglobin content in plasma, a marker of hemolytic processes, is significantly lower and the assay should be done by more elaborative techniques. In this paper, piezoelectric biosensor containing QCM platform with basic frequency of oscillations 10 MHz and gold circle electrodes on the opposite sites, antibody against hemoglobin and iron oxide nanoparticles as blocker and antibody layer stabilizer was prepared. The assay exerted limit of detection equal to 0.063 mg/ml and the calibration well correlated (r(2)= 0.995) with standard ELISA. No interference by immunoglobulins and albumin was proved. The assay appears to applicable for clinical praxis and outside of central hospital laboratories. Reasonably limit of detection, low price of the biosensor and device, simple procedure of sample use, no necessity of specific reagents and label free type of assay are the major advantages of the biosensor.

Název v anglickém jazyce

Construction of a QCM Biosensor for free Hemoglobin Assay

Popis výsledku anglicky

Protein responsible for oxygen transfer, hemoglobin, is an important marker in biochemistry that can be measured in blood and blood plasma. Meaning of the assays are different in the two fluids. While blood hemoglobin serving as a marker of anemia and some oncotic processes is simply measurable by spectroscopy, hemoglobin content in plasma, a marker of hemolytic processes, is significantly lower and the assay should be done by more elaborative techniques. In this paper, piezoelectric biosensor containing QCM platform with basic frequency of oscillations 10 MHz and gold circle electrodes on the opposite sites, antibody against hemoglobin and iron oxide nanoparticles as blocker and antibody layer stabilizer was prepared. The assay exerted limit of detection equal to 0.063 mg/ml and the calibration well correlated (r(2)= 0.995) with standard ELISA. No interference by immunoglobulins and albumin was proved. The assay appears to applicable for clinical praxis and outside of central hospital laboratories. Reasonably limit of detection, low price of the biosensor and device, simple procedure of sample use, no necessity of specific reagents and label free type of assay are the major advantages of the biosensor.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

1452-3981

Svazek periodika

14

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

10

Strana od-do

5237-5246

Kód UT WoS článku

000470877100027

EID výsledku v databázi Scopus

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