Piezoelectric Immunosensor for the Determination of C-Reactive Protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00537181" target="_blank" >RIV/60162694:G44__/19:00537181 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol14/140908470.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol14/140908470.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2019.09.02" target="_blank" >10.20964/2019.09.02</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Piezoelectric Immunosensor for the Determination of C-Reactive Protein

Popis výsledku v původním jazyce

C-reactive protein (CRP) is a common biomarker of inflammatory processes and it typically serves for the distinguishing of bacterial and viral diseases. It is typically measured in the blood, plasma or serum by immunoassay like agglutination or enzymatic immunoassays. In this paper, a new immunoassay based on a piezoelectric biosensor was introduced. QCM with basic frequency of oscillations 10 MHz and gold electrodes served as a platform and it was modified with antibodies against CRP and silica nanoparticles. Separately, antibody functionalized silica nanoparticles were prepared and they further served for signal amplification. CRP was assayed with limit of detection equal to 0.080 mg/l. The assay was successfully validated to standard ELISA method and no significant interference by IgM, TNF alpha and albumin was observed. The biosensor-based assay appears to be practically applicable and can serve for routine diagnosis purpose.

Název v anglickém jazyce

Piezoelectric Immunosensor for the Determination of C-Reactive Protein

Popis výsledku anglicky

C-reactive protein (CRP) is a common biomarker of inflammatory processes and it typically serves for the distinguishing of bacterial and viral diseases. It is typically measured in the blood, plasma or serum by immunoassay like agglutination or enzymatic immunoassays. In this paper, a new immunoassay based on a piezoelectric biosensor was introduced. QCM with basic frequency of oscillations 10 MHz and gold electrodes served as a platform and it was modified with antibodies against CRP and silica nanoparticles. Separately, antibody functionalized silica nanoparticles were prepared and they further served for signal amplification. CRP was assayed with limit of detection equal to 0.080 mg/l. The assay was successfully validated to standard ELISA method and no significant interference by IgM, TNF alpha and albumin was observed. The biosensor-based assay appears to be practically applicable and can serve for routine diagnosis purpose.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

1452-3981

Svazek periodika

14

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

9

Strana od-do

8470-8478

Kód UT WoS článku

000483307100010

EID výsledku v databázi Scopus

2-s2.0-85072854723