Immunoassay of interferon gamma by quartz crystal microbalance biosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556106" target="_blank" >RIV/60162694:G44__/20:00556106 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0039914020304586?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914020304586?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2020.121167" target="_blank" >10.1016/j.talanta.2020.121167</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunoassay of interferon gamma by quartz crystal microbalance biosensor

Popis výsledku v původním jazyce

Interferon gamma (IFN gamma) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFN gamma are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor working on the principle of quartz crystal microbalance (QCM) was developed as an alternative to the standard analytical methods for IFN gamma. The biosensor contained antibodies against IFN gamma immobilized on QCM and also on gold nanoparticles. A sandwich containing QCM, gold nanoparticles and IFN gamma was formed and formation of the sandwich caused decrease of oscillation frequency. The assay exerted limit of detection 5.7 pg/ml for a sample sized 50 mu l and one measuring cycle was finished within 90 min. The assay by biosensor fully correlated to standard ELISA. In a conclusion, the biosensor appears to be a fully applicable analytical tool for a simple assay of IFN gamma. Overall simplicity and no special requirement on staff and equipment are the major advantages of the here presented assay.

Název v anglickém jazyce

Immunoassay of interferon gamma by quartz crystal microbalance biosensor

Popis výsledku anglicky

Interferon gamma (IFN gamma) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFN gamma are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor working on the principle of quartz crystal microbalance (QCM) was developed as an alternative to the standard analytical methods for IFN gamma. The biosensor contained antibodies against IFN gamma immobilized on QCM and also on gold nanoparticles. A sandwich containing QCM, gold nanoparticles and IFN gamma was formed and formation of the sandwich caused decrease of oscillation frequency. The assay exerted limit of detection 5.7 pg/ml for a sample sized 50 mu l and one measuring cycle was finished within 90 min. The assay by biosensor fully correlated to standard ELISA. In a conclusion, the biosensor appears to be a fully applicable analytical tool for a simple assay of IFN gamma. Overall simplicity and no special requirement on staff and equipment are the major advantages of the here presented assay.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

218

Číslo periodika v rámci svazku

Oct

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

121167

Kód UT WoS článku

000564486000009

EID výsledku v databázi Scopus

2-s2.0-85085258542