QCM biosensor for Prostate Specific Antigen assay using antibody - gold particle conjugate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557067" target="_blank" >RIV/60162694:G44__/21:00557067 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/abstracts/vol16/21051.pdf" target="_blank" >http://www.electrochemsci.org/abstracts/vol16/21051.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2021.05.04" target="_blank" >10.20964/2021.05.04</a>

Jazyk výsledku

angličtina

Název v původním jazyce

QCM biosensor for Prostate Specific Antigen assay using antibody - gold particle conjugate

Popis výsledku v původním jazyce

Prostate Specific Antigen (PSA) is a key marker for prostate cancer screening by standard laboratory tests based on blood, plasma or serum analysis. The current laboratory methods for PSA level determination are based on various chromatographic, immunochemical and other instrumental analyses that has to be done in the specialized laboratories. Experiments in this work are focused on construction of a Quartz Crystal Microbalance (QCM) biosensor as a simple device suitable for point-of-care diagnosis based on PSA concentration determination in body fluids. The QCM sensors were covered with antibody against PSA and gold nanoparticles modified with specific antibody against PSA were prepared in a separate step. Change in oscillation frequency was measured as the immunocomplexes QCM biosensor - PSA - modified gold nanoparticles were formed. Calibrating for standard solution of PSA, limit of detection equal to 0.054 mu g/l and limit of quantification equal to 0.18 mu g/l were figured. The biosensor was not sensitive to interference of other plasma proteins or to matrix effect and fully correlated with the Enzyme-Linked Immunosorbent Assay (ELISA) as a standard method. In a conclusion, the biosensor appears to be a simple and reliable tool that can be used in standard laboratory and point-of-care testing.

Název v anglickém jazyce

QCM biosensor for Prostate Specific Antigen assay using antibody - gold particle conjugate

Popis výsledku anglicky

Prostate Specific Antigen (PSA) is a key marker for prostate cancer screening by standard laboratory tests based on blood, plasma or serum analysis. The current laboratory methods for PSA level determination are based on various chromatographic, immunochemical and other instrumental analyses that has to be done in the specialized laboratories. Experiments in this work are focused on construction of a Quartz Crystal Microbalance (QCM) biosensor as a simple device suitable for point-of-care diagnosis based on PSA concentration determination in body fluids. The QCM sensors were covered with antibody against PSA and gold nanoparticles modified with specific antibody against PSA were prepared in a separate step. Change in oscillation frequency was measured as the immunocomplexes QCM biosensor - PSA - modified gold nanoparticles were formed. Calibrating for standard solution of PSA, limit of detection equal to 0.054 mu g/l and limit of quantification equal to 0.18 mu g/l were figured. The biosensor was not sensitive to interference of other plasma proteins or to matrix effect and fully correlated with the Enzyme-Linked Immunosorbent Assay (ELISA) as a standard method. In a conclusion, the biosensor appears to be a simple and reliable tool that can be used in standard laboratory and point-of-care testing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

9

Strana od-do

21051

Kód UT WoS článku

000661483700001

EID výsledku v databázi Scopus

2-s2.0-85104748474