Heat Shock Protein 60 (HSP60) detection by QCM Biosensor and Antibody Covered Gold Nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557068" target="_blank" >RIV/60162694:G44__/21:00557068 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol16/210512.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol16/210512.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2021.05.11" target="_blank" >10.20964/2021.05.11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heat Shock Protein 60 (HSP60) detection by QCM Biosensor and Antibody Covered Gold Nanoparticles

Popis výsledku v původním jazyce

Heat Shock Protein 60 (HSP60) is a 60 kDa weighting chaperonin that is an evolutionary conserved protein occurring in a wide number of organisms. It can serve as a plasma or blood serum biomarker of serious pathologies including cancer. In this work, quartz crystal microbalance (QCM) biosensor was constructed as a tool for a simple measurement of HSP 60 level. The QCMs were covered with an antibody specific to HSP60 and the antibody modified gold nanoparticles were also prepared. The assay was based of sandwich forming with captured HSP60, presence of HSP60 caused decrease of QCM oscillation frequency. The described assay exerted good sensitivity to HSP60, limit of detection for the assay was equal to 83 pg/ml for a sample with volume 5 mu l, one assay cycle was finished within 90 minutes. The assay well correlated to standard Enzyme-Linked ImmunoSorbent Assay (ELISA) with coefficient of determination 0.985 and was not sensitive to interference by selected proteins (HSP70, albumin, immunoglobulins). In a conclusion, the biosensor appears to be a reliable tool for the detection of HSP60, the achieved specifications make it promising for a routine application.

Název v anglickém jazyce

Heat Shock Protein 60 (HSP60) detection by QCM Biosensor and Antibody Covered Gold Nanoparticles

Popis výsledku anglicky

Heat Shock Protein 60 (HSP60) is a 60 kDa weighting chaperonin that is an evolutionary conserved protein occurring in a wide number of organisms. It can serve as a plasma or blood serum biomarker of serious pathologies including cancer. In this work, quartz crystal microbalance (QCM) biosensor was constructed as a tool for a simple measurement of HSP 60 level. The QCMs were covered with an antibody specific to HSP60 and the antibody modified gold nanoparticles were also prepared. The assay was based of sandwich forming with captured HSP60, presence of HSP60 caused decrease of QCM oscillation frequency. The described assay exerted good sensitivity to HSP60, limit of detection for the assay was equal to 83 pg/ml for a sample with volume 5 mu l, one assay cycle was finished within 90 minutes. The assay well correlated to standard Enzyme-Linked ImmunoSorbent Assay (ELISA) with coefficient of determination 0.985 and was not sensitive to interference by selected proteins (HSP70, albumin, immunoglobulins). In a conclusion, the biosensor appears to be a reliable tool for the detection of HSP60, the achieved specifications make it promising for a routine application.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

10

Strana od-do

210512

Kód UT WoS článku

000661483700012

EID výsledku v databázi Scopus

2-s2.0-85104728502