QCM immunosensor for the determination of Staphylococcus aureus antigen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555659" target="_blank" >RIV/60162694:G44__/20:00555659 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs11696-019-00889-5" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-019-00889-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-019-00889-5" target="_blank" >10.1007/s11696-019-00889-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

QCM immunosensor for the determination of Staphylococcus aureus antigen

Popis výsledku v původním jazyce

Staphylococcus aureus is a causative agent of infectious diseases and a producer of a group of highly toxic proteins called Staphylococcal enterotoxins. Currently, the presence of S. aureus in a sample can be determined by a wide spectrum of genetic, immunochemical and microbiological techniques. Despite availability of standard methods and devices, they are not fully suitable for a simple, label-free mode of use by an unskilled worker. In this paper, a biosensor based on quartz crystal microbalance (QCM) sensor is performed as a tool for a simple and reliable determination of S. aureus. The biosensor contained an antibody specific to S. aureus immobilized through protein A on 10 MHz QCM sensors and the interdigitated layer was stabilized by iron nanoparticles. The assay was performed as label free and sample was applied without any pretreatment. Limit of detection 145 mu g/ml (5.18 x 10(8) CFU/ml) was achieved. When considered standard size of a sample 50 mu l, the limit of detection was equal to 7.25 mu g (2.59 x -10(7) CFU). The biosensors kept more than 90% sensitivity to the analyte after storage in laboratory conditions for 90 days. Good correlation to standard enzyme-linked immunosorbent assay was also proved. The biosensor appears as a simple but reliable tool for S. aureus assay and the QCM platform would provide further applications in microbial analyses.

Název v anglickém jazyce

QCM immunosensor for the determination of Staphylococcus aureus antigen

Popis výsledku anglicky

Staphylococcus aureus is a causative agent of infectious diseases and a producer of a group of highly toxic proteins called Staphylococcal enterotoxins. Currently, the presence of S. aureus in a sample can be determined by a wide spectrum of genetic, immunochemical and microbiological techniques. Despite availability of standard methods and devices, they are not fully suitable for a simple, label-free mode of use by an unskilled worker. In this paper, a biosensor based on quartz crystal microbalance (QCM) sensor is performed as a tool for a simple and reliable determination of S. aureus. The biosensor contained an antibody specific to S. aureus immobilized through protein A on 10 MHz QCM sensors and the interdigitated layer was stabilized by iron nanoparticles. The assay was performed as label free and sample was applied without any pretreatment. Limit of detection 145 mu g/ml (5.18 x 10(8) CFU/ml) was achieved. When considered standard size of a sample 50 mu l, the limit of detection was equal to 7.25 mu g (2.59 x -10(7) CFU). The biosensors kept more than 90% sensitivity to the analyte after storage in laboratory conditions for 90 days. Good correlation to standard enzyme-linked immunosorbent assay was also proved. The biosensor appears as a simple but reliable tool for S. aureus assay and the QCM platform would provide further applications in microbial analyses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

2585-7290

e-ISSN

1336-9075

Svazek periodika

74

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

451-458

Kód UT WoS článku

000514312600007

EID výsledku v databázi Scopus

2-s2.0-85069875607