QCM Biosensor for Measurement of Glycated Hemoglobin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00555439" target="_blank" >RIV/60162694:G44__/19:00555439 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol14/141211340.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol14/141211340.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2019.12.16" target="_blank" >10.20964/2019.12.16</a>

Jazyk výsledku

angličtina

Název v původním jazyce

QCM Biosensor for Measurement of Glycated Hemoglobin

Popis výsledku v původním jazyce

In the body, glycated hemoglobin is spontaneously created by a reaction between hemoglobin and glucose. The glycation reaches only minimal scale in health people but it is much higher as the glucose level is increased. It typically serves as a marker in diabetes mellitus. Chromatography, mass spectrometry and immunoassays are the standard methods for glycated hemoglobin measurement in the current clinical praxis. In this work, piezoelectric biosensor is proposed as a tool for glycated hemoglobin measurement. The biosensor contained immobilized antibodies against glycated hemoglobin in a stable film composed from iron oxide nanoparticles. Standard solution of glycated hemoglobin and human blood samples were used for the prepared biosensor characterization. Non glycated hemoglobin and albumin served for interference testing. The biosensor exerted quite good limit of detection highly under expected physiological level: 0.045 mg/ml and coefficient of determination 0.968 for the assay was achieved. The assay by biosensor fully correlated to standard ELISA (coefficient of determination 0.948). Non-glycated hemoglobin and albumin in concentrations expected in blood or blood plasma did not caused interference in the assay. The piezoelectric biosensor seems to be a promising tool for routine assay of glycated hemoglobin. Due to overall simplicity, no application of specific reagents or sample manipulation, the biosensor can be easily used in field conditions or in the environment of small medical facilities.

Název v anglickém jazyce

QCM Biosensor for Measurement of Glycated Hemoglobin

Popis výsledku anglicky

In the body, glycated hemoglobin is spontaneously created by a reaction between hemoglobin and glucose. The glycation reaches only minimal scale in health people but it is much higher as the glucose level is increased. It typically serves as a marker in diabetes mellitus. Chromatography, mass spectrometry and immunoassays are the standard methods for glycated hemoglobin measurement in the current clinical praxis. In this work, piezoelectric biosensor is proposed as a tool for glycated hemoglobin measurement. The biosensor contained immobilized antibodies against glycated hemoglobin in a stable film composed from iron oxide nanoparticles. Standard solution of glycated hemoglobin and human blood samples were used for the prepared biosensor characterization. Non glycated hemoglobin and albumin served for interference testing. The biosensor exerted quite good limit of detection highly under expected physiological level: 0.045 mg/ml and coefficient of determination 0.968 for the assay was achieved. The assay by biosensor fully correlated to standard ELISA (coefficient of determination 0.948). Non-glycated hemoglobin and albumin in concentrations expected in blood or blood plasma did not caused interference in the assay. The piezoelectric biosensor seems to be a promising tool for routine assay of glycated hemoglobin. Due to overall simplicity, no application of specific reagents or sample manipulation, the biosensor can be easily used in field conditions or in the environment of small medical facilities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

1452-3981

Svazek periodika

14

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

9

Strana od-do

11340-11348

Kód UT WoS článku

000502341900052

EID výsledku v databázi Scopus

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