The determination of human albumin by a quartz crystal microbalance immunosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889860" target="_blank" >RIV/60162694:G44__/18:43889860 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol13/130908471.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol13/130908471.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2018.09.14" target="_blank" >10.20964/2018.09.14</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The determination of human albumin by a quartz crystal microbalance immunosensor

Popis výsledku v původním jazyce

Albumin is a protein serving as a standard biochemical marker which is assayed in the blood and blood plasma as a liver function test but its diagnostics importance is much wider. In the current time, albumin can be determined by standard immunochemical and spectral methods. In this work, immunosensor based on the piezoelectric10 MHz QCM was developed to determine albumin in levels corresponding with expected concentration in blood plasma. The immunosensor contained an antibody specific to albumin interdigitated to electrode surface through protein A. The immunosensor was performed for the determination of albumin and limit of detection 0.234 mg/ml was achieved. The limit of detection is lower than the expected plasma level of albumin (35 – 55 mg/ml). The immunosensor assay fully correlated with the standard ELISA and the immunosensor exerted good long-term stability for at least two months. The immunosensor based determination of albumin appears to be a promising tool for practical use in field or home care conditions. No elaborative manipulation with samples or necessity to use specific reagents is a significant advantage of the immunosensor based assay.

Název v anglickém jazyce

The determination of human albumin by a quartz crystal microbalance immunosensor

Popis výsledku anglicky

Albumin is a protein serving as a standard biochemical marker which is assayed in the blood and blood plasma as a liver function test but its diagnostics importance is much wider. In the current time, albumin can be determined by standard immunochemical and spectral methods. In this work, immunosensor based on the piezoelectric10 MHz QCM was developed to determine albumin in levels corresponding with expected concentration in blood plasma. The immunosensor contained an antibody specific to albumin interdigitated to electrode surface through protein A. The immunosensor was performed for the determination of albumin and limit of detection 0.234 mg/ml was achieved. The limit of detection is lower than the expected plasma level of albumin (35 – 55 mg/ml). The immunosensor assay fully correlated with the standard ELISA and the immunosensor exerted good long-term stability for at least two months. The immunosensor based determination of albumin appears to be a promising tool for practical use in field or home care conditions. No elaborative manipulation with samples or necessity to use specific reagents is a significant advantage of the immunosensor based assay.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/TJ01000478" target="_blank" >TJ01000478: Konstrukce senzoru pro kvantitativní stanovení protilátek proti tkáňové transglutaminase pro diagnostiku celiakie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

10

Strana od-do

8471-8480

Kód UT WoS článku

000452562400014

EID výsledku v databázi Scopus

2-s2.0-85052612244