Piezoelectric biosensor for the determination of Tumor Necrosis Factor Alpha

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889330" target="_blank" >RIV/60162694:G44__/18:43889330 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0039914017310731" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0039914017310731</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2017.10.031" target="_blank" >10.1016/j.talanta.2017.10.031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Piezoelectric biosensor for the determination of Tumor Necrosis Factor Alpha

Popis výsledku v původním jazyce

Tumor Necrosis Factor Alpha (TNF alpha) is an important marker of inflammatory processes in human body. In the current healthcare, determination of TNFa blood or plasma level is done by Enzyme Linked Immuno-Sorbent Assay (ELISA) as a primary choice method. Piezoelectric immunosensors are analytical platform recording affinity interactions on their surface. It is inferred that the immunosensors would be a functional alternative to the ELISA. In this study, antibody against TNF alpha was immobilized on Quartz Crystal Microbalance (QCM) sensor and the same was made on magnetic particles. Human TNFa was measured in a way of interaction with QCM surface and then the particles were applied. The assay exerted sufficient limit of detection equal to 1.62 pg/ml and it also fully correlated with standard ELISA tests. No interference by interleukin 6 or albumin was observed. Long term stability of the immunosensors lasting for at least three months was found. The immunosensors appears to be readily for practical performance and it would be an alternative to the standard ELISA especially when diagnoses made in field, homecare conditions or conditions of small hospitals as an emergency test.

Název v anglickém jazyce

Piezoelectric biosensor for the determination of Tumor Necrosis Factor Alpha

Popis výsledku anglicky

Tumor Necrosis Factor Alpha (TNF alpha) is an important marker of inflammatory processes in human body. In the current healthcare, determination of TNFa blood or plasma level is done by Enzyme Linked Immuno-Sorbent Assay (ELISA) as a primary choice method. Piezoelectric immunosensors are analytical platform recording affinity interactions on their surface. It is inferred that the immunosensors would be a functional alternative to the ELISA. In this study, antibody against TNF alpha was immobilized on Quartz Crystal Microbalance (QCM) sensor and the same was made on magnetic particles. Human TNFa was measured in a way of interaction with QCM surface and then the particles were applied. The assay exerted sufficient limit of detection equal to 1.62 pg/ml and it also fully correlated with standard ELISA tests. No interference by interleukin 6 or albumin was observed. Long term stability of the immunosensors lasting for at least three months was found. The immunosensors appears to be readily for practical performance and it would be an alternative to the standard ELISA especially when diagnoses made in field, homecare conditions or conditions of small hospitals as an emergency test.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

178

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

970-973

Kód UT WoS článku

000416615500129

EID výsledku v databázi Scopus

2-s2.0-85032383725