Determination of Hypericin in Hypericum perforatum (St. John's Wort) Using Classical C18 and Pentafluorophenyl Stationary Phases: Contribution of Pi-Pi Interactions to High-Performance Liquid Chromatography (HPLC)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00536969" target="_blank" >RIV/60162694:G44__/19:00536969 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62690094:18450/19:50015576 RIV/62690094:18470/19:50015576 RIV/00216208:11160/19:10394584 RIV/00179906:_____/19:10394584

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/00032719.2019.1571076?journalCode=lanl20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/00032719.2019.1571076?journalCode=lanl20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2019.1571076" target="_blank" >10.1080/00032719.2019.1571076</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Hypericin in Hypericum perforatum (St. John's Wort) Using Classical C18 and Pentafluorophenyl Stationary Phases: Contribution of Pi-Pi Interactions to High-Performance Liquid Chromatography (HPLC)

Popis výsledku v původním jazyce

Hypericin is one of the most important bioactive substances present in Hypericum perforatum (Saint John's Wort), which exhibits various biological activities such as inhibition of neuronal processes coupled with transmission of serotonin, dopamine, gamma-amino butyric acid and L-glutamine. This red-colored derivative of anthraquinone contains six benzene cycles and four hydroxy groups that polarize electron density in the conjugated double bond system and enable the molecule to act as a Lewis base in charge-transfer complexes. In this study, four stationary phases modified by the pentafluorophenyl group were selected to investigate the contribution of pi-pi interactions to the improvement of hypericin separation in comparison to the separation provided with a classical C18 based chromatographic column. In order to develop a suitable gradient chromatographic method for the quantification of hypericin, normal, polar organic and reverse elution modes were evaluated using high-performance liquid chromatography with ultraviolet-visible and mass spectrometry detection. It was disclosed in the analyses that the pentafluorophenyl stationary phase can separate hypericin from hyperforin, which was not possible to achieve with the C18 based stationary phase. The best analytical method found, employing the pentafluorophenyl stationary phase, showed sufficient linearity, accuracy and precision and was used for the determination of hypericin in Saint John's Wort.

Název v anglickém jazyce

Determination of Hypericin in Hypericum perforatum (St. John's Wort) Using Classical C18 and Pentafluorophenyl Stationary Phases: Contribution of Pi-Pi Interactions to High-Performance Liquid Chromatography (HPLC)

Popis výsledku anglicky

Hypericin is one of the most important bioactive substances present in Hypericum perforatum (Saint John's Wort), which exhibits various biological activities such as inhibition of neuronal processes coupled with transmission of serotonin, dopamine, gamma-amino butyric acid and L-glutamine. This red-colored derivative of anthraquinone contains six benzene cycles and four hydroxy groups that polarize electron density in the conjugated double bond system and enable the molecule to act as a Lewis base in charge-transfer complexes. In this study, four stationary phases modified by the pentafluorophenyl group were selected to investigate the contribution of pi-pi interactions to the improvement of hypericin separation in comparison to the separation provided with a classical C18 based chromatographic column. In order to develop a suitable gradient chromatographic method for the quantification of hypericin, normal, polar organic and reverse elution modes were evaluated using high-performance liquid chromatography with ultraviolet-visible and mass spectrometry detection. It was disclosed in the analyses that the pentafluorophenyl stationary phase can separate hypericin from hyperforin, which was not possible to achieve with the C18 based stationary phase. The best analytical method found, employing the pentafluorophenyl stationary phase, showed sufficient linearity, accuracy and precision and was used for the determination of hypericin in Saint John's Wort.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Letters

ISSN

0003-2719

e-ISSN

1532-236X

Svazek periodika

52

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

25

Strana od-do

1788-1812

Kód UT WoS článku

000466589500007

EID výsledku v databázi Scopus

2-s2.0-85062354921