New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 2. Determination of cis/trans- isomers -iso- -acids in beer using Ultra Performance Liquid Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F12%3A%230000695" target="_blank" >RIV/60193697:_____/12:#0000695 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.kvasnyprumysl.cz/en/journal/2012/4/" target="_blank" >http://www.kvasnyprumysl.cz/en/journal/2012/4/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 2. Determination of cis/trans- isomers -iso- -acids in beer using Ultra Performance Liquid Chromatography

Popis výsledku v původním jazyce

The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 ?m particles, was tested for the separation and determination of steric forms of iso-?-acids in beer. The new UHPLC method was compared with the HPLC method, which is used for iso-?-acid determinations in RIBM. The comparison was performed with respect to the speed of analysis, separation efficiency, mobile phase consumption, and finally repeatability. The results of this study determined all the expected advantages of the UHPLC; while maintaining the resolution this method reduced analysis time 2.5 times compared to the previous HPLC. In addition, the UHPLC reduces the consumption of organic solvents 10 times. Apart from the economic benefits theUHPLC provides a high precision of the measurement, which was verified as repeatability of the UHPLC for both forms of iso-?-aids. Especially the trans-iso-?-isomers are significant indicators of beer aging during storage and transport. I

Název v anglickém jazyce

New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 2. Determination of cis/trans- isomers -iso- -acids in beer using Ultra Performance Liquid Chromatography

Popis výsledku anglicky

The Ultra High Performance Liquid Chromatography (UHPLC), which uses the principle of separation on sub-2 ?m particles, was tested for the separation and determination of steric forms of iso-?-acids in beer. The new UHPLC method was compared with the HPLC method, which is used for iso-?-acid determinations in RIBM. The comparison was performed with respect to the speed of analysis, separation efficiency, mobile phase consumption, and finally repeatability. The results of this study determined all the expected advantages of the UHPLC; while maintaining the resolution this method reduced analysis time 2.5 times compared to the previous HPLC. In addition, the UHPLC reduces the consumption of organic solvents 10 times. Apart from the economic benefits theUHPLC provides a high precision of the measurement, which was verified as repeatability of the UHPLC for both forms of iso-?-aids. Especially the trans-iso-?-isomers are significant indicators of beer aging during storage and transport. I

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Kvasný průmysl

ISSN

0023-5830

e-ISSN

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Svazek periodika

58

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

94-99

Kód UT WoS článku

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EID výsledku v databázi Scopus

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