Optimisation of extraction method for determination of PAHs in malt using HPLC-FLD method
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F21%3AN0000058" target="_blank" >RIV/60193697:_____/21:N0000058 - isvavai.cz</a>
Výsledek na webu
<a href="http://ceskachromatografickaskola.cz/admin/Include/Data/getfile.php?id_soubor=56&db=2-ceskachro" target="_blank" >http://ceskachromatografickaskola.cz/admin/Include/Data/getfile.php?id_soubor=56&db=2-ceskachro</a>
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Optimisation of extraction method for determination of PAHs in malt using HPLC-FLD method
Popis výsledku v původním jazyce
The aim of this study was to develop a practical method for the determination of 15 polycyclic aromatic hydrocompounds (PAHs) in the samples of barley malt. Two extraction methods were compared; QuEChERS method and the pressurized extraction by acidified acetonitrile performed by EDGE extractor. Subsequentely the clean-up by dSPE method was performed (PSA and Supel™ QuE Z-Sep in the case of QuEChERS method and SPE PSA tubes in the case of the EDGE extractor). The chromatographic analysis was carried out by HPLC with fluorescence detection. The two methods were compared using spiked malt specimens. The recovery of individual PAHs was used for the comparison of both methods. Considering generally better recovery of the analysed compounds with the use of QuEChERS method (Recovery ranged from 85 to 134 %), its simplicity, price, and material availability the QuECHers method was selected as more convenient for routine determination of PAHs in malt matrix. The values of PAH content in samples of common malt were under or close to the LOQ values. The PAH residues over the LOQ values were found in samples of smoked malt.Poster, Česká chromatografická škola - HPLC.cz 2021, 19. - 22. 9. 2021 Zaječí.
Název v anglickém jazyce
Optimisation of extraction method for determination of PAHs in malt using HPLC-FLD method
Popis výsledku anglicky
The aim of this study was to develop a practical method for the determination of 15 polycyclic aromatic hydrocompounds (PAHs) in the samples of barley malt. Two extraction methods were compared; QuEChERS method and the pressurized extraction by acidified acetonitrile performed by EDGE extractor. Subsequentely the clean-up by dSPE method was performed (PSA and Supel™ QuE Z-Sep in the case of QuEChERS method and SPE PSA tubes in the case of the EDGE extractor). The chromatographic analysis was carried out by HPLC with fluorescence detection. The two methods were compared using spiked malt specimens. The recovery of individual PAHs was used for the comparison of both methods. Considering generally better recovery of the analysed compounds with the use of QuEChERS method (Recovery ranged from 85 to 134 %), its simplicity, price, and material availability the QuECHers method was selected as more convenient for routine determination of PAHs in malt matrix. The values of PAH content in samples of common malt were under or close to the LOQ values. The PAH residues over the LOQ values were found in samples of smoked malt.Poster, Česká chromatografická škola - HPLC.cz 2021, 19. - 22. 9. 2021 Zaječí.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů