Optimizing the extraction and clean-up step for determination of polycyclic aromatic hydrocarbons in malt using HPLC-FLD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F23%3AN0000083" target="_blank" >RIV/60193697:_____/23:N0000083 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10452275

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12161-022-02405-0" target="_blank" >https://link.springer.com/article/10.1007/s12161-022-02405-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-022-02405-0" target="_blank" >10.1007/s12161-022-02405-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimizing the extraction and clean-up step for determination of polycyclic aromatic hydrocarbons in malt using HPLC-FLD

Popis výsledku v původním jazyce

This study presents an optimization and validation of a new method for the determination of 12 polycyclic aromatic hydrocarbons (PAHs) in barley malt samples. The sample preparation operates on the principle of a Quick, Easy, Cheap, Efficient, Rugged, and Safe (QuEChERS) extraction method. Lipidic compounds are then frozen out and finally cleared away by an additional dSPE clean-up. For this reason, the mixture of primary secondary amine (PSA) and zirconium dioxide-based sorbent (Z-Sep) granted the most efficient choice. A comparison with a more conventional extraction method and agreement with the EC Regulations confirmed the aptness of our new method. The comparison and validation were carried out using a spiked malt sample. The recoveries ranged from 84 to 134% and LOQs from 0.3 to 0.7 mu g/kg. The analysis alone was performed by HPLC with fluorescence detection. This approach provides not only satisfactory results but also an opportunity to prepare more samples at once in a relatively short time.

Název v anglickém jazyce

Optimizing the extraction and clean-up step for determination of polycyclic aromatic hydrocarbons in malt using HPLC-FLD

Popis výsledku anglicky

This study presents an optimization and validation of a new method for the determination of 12 polycyclic aromatic hydrocarbons (PAHs) in barley malt samples. The sample preparation operates on the principle of a Quick, Easy, Cheap, Efficient, Rugged, and Safe (QuEChERS) extraction method. Lipidic compounds are then frozen out and finally cleared away by an additional dSPE clean-up. For this reason, the mixture of primary secondary amine (PSA) and zirconium dioxide-based sorbent (Z-Sep) granted the most efficient choice. A comparison with a more conventional extraction method and agreement with the EC Regulations confirmed the aptness of our new method. The comparison and validation were carried out using a spiked malt sample. The recoveries ranged from 84 to 134% and LOQs from 0.3 to 0.7 mu g/kg. The analysis alone was performed by HPLC with fluorescence detection. This approach provides not only satisfactory results but also an opportunity to prepare more samples at once in a relatively short time.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

1936-976X

Svazek periodika

16

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

168-176

Kód UT WoS článku

000854676600001

EID výsledku v databázi Scopus

2-s2.0-85138309032