Determination of glyphosate in beer and raw beer materials

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F23%3AN0000065" target="_blank" >RIV/60193697:_____/23:N0000065 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of glyphosate in beer and raw beer materials

Popis výsledku v původním jazyce

Widespread use of pesticides in the agricultural industry leads to the presence of their residues in soils, water sources and plants. Glyphosate is the most famous and most used herbicide. This compound is the main component of the Roundup® herbicide. This herbicide inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which is an enzyme of the aromatic acid biosynthetic pathway. This inhibition prevents synthesis of essential aromatic acids which are needed for protein biosynthesis. This herbicide is absorbed by foliage of the plant and transferred through the plant via vascular bundles. Soil microorganisms can metabolize this herbicide into aminomethylphosphoric acid (AMPA). [1] The aim of this study was to develop, optimize and validate a method based on derivatization of glyphosate with fluorenylmethyloxycarbonyl chloride (FMOC-Cl), subsequent cleanup with Oasis® PRiME HLB 6cc Extraction Cartridges and determination with liquid chromatography coupled with mass spectrometry. Pesticide limits are set in the European Union according to Regulation (EC) No. 396/2005 of the European Parliament and Council on maximum limits for pesticide residues in food and feed of plant and animal origin. The European Food Safety Authority (EFSA) established in 2019 the maximum residue levels for the sum of glyphosate and its metabolites which include AMPA. Pesticides residues are persistent problem in agricultural industry. These pesticides can be transferred from raw materials into final products which include beer. This method for glyphosate determination can be used to monitor its content in beer and raw beer materials such as barley. Results were presented in the form of a poster at the conference 15. Trends in Brewing, Ghent, 2-6 April 2023.

Název v anglickém jazyce

Determination of glyphosate in beer and raw beer materials

Popis výsledku anglicky

Widespread use of pesticides in the agricultural industry leads to the presence of their residues in soils, water sources and plants. Glyphosate is the most famous and most used herbicide. This compound is the main component of the Roundup® herbicide. This herbicide inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which is an enzyme of the aromatic acid biosynthetic pathway. This inhibition prevents synthesis of essential aromatic acids which are needed for protein biosynthesis. This herbicide is absorbed by foliage of the plant and transferred through the plant via vascular bundles. Soil microorganisms can metabolize this herbicide into aminomethylphosphoric acid (AMPA). [1] The aim of this study was to develop, optimize and validate a method based on derivatization of glyphosate with fluorenylmethyloxycarbonyl chloride (FMOC-Cl), subsequent cleanup with Oasis® PRiME HLB 6cc Extraction Cartridges and determination with liquid chromatography coupled with mass spectrometry. Pesticide limits are set in the European Union according to Regulation (EC) No. 396/2005 of the European Parliament and Council on maximum limits for pesticide residues in food and feed of plant and animal origin. The European Food Safety Authority (EFSA) established in 2019 the maximum residue levels for the sum of glyphosate and its metabolites which include AMPA. Pesticides residues are persistent problem in agricultural industry. These pesticides can be transferred from raw materials into final products which include beer. This method for glyphosate determination can be used to monitor its content in beer and raw beer materials such as barley. Results were presented in the form of a poster at the conference 15. Trends in Brewing, Ghent, 2-6 April 2023.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů