Changes of isoflavonoid levels in pea (Pisum sativum L.) seeds, seedlings and plants influenced by UV-A and gamma-irradiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F01%3A00002553" target="_blank" >RIV/60460709:41210/01:00002553 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Changes of isoflavonoid levels in pea (Pisum sativum L.) seeds, seedlings and plants influenced by UV-A and gamma-irradiation

Popis výsledku v původním jazyce

In two pea cultivars (Lantra, Menhir) changes in isoflavonoid levels caused by UV-A and g - irradiation were investigated. The changes were determined in seeds, seedlings and plants. UV-A irradiation was performed for 0 (control), 24, 48 and 72 hours atl=365.5 nm, P= 125 W, g - irradiation with 60Co at doses 0 (control), 10, 20 and 40 Gy. Cultivars responded differentially to various doses of irradiation in both types of irradiation. Daidzein and genistein are the main isoflavones of biological interest belonging to the group of naturally occurring isoflavonoid phytoestrogens. Daidzein (Dai) and genistein (Gen) were determinated by a selective and sensitive radioimmunoassay 7- and 4´- cross-reactive method. In less amounts was determined biochanin A (Bio A) by means of 7-cross-reactivity. The highest levels among isoflavonoids were found for daidzein-7 cross-reactivity in both pea varieties (in average in Lantra seeds 6 562.4 mg kg-1 DM, in Menhir seeds 3 481.3 mg kg-1 DM). During spr

Název v anglickém jazyce

Changes of isoflavonoid levels in pea (Pisum sativum L.) seeds, seedlings and plants influenced by UV-A and gamma-irradiation

Popis výsledku anglicky

In two pea cultivars (Lantra, Menhir) changes in isoflavonoid levels caused by UV-A and g - irradiation were investigated. The changes were determined in seeds, seedlings and plants. UV-A irradiation was performed for 0 (control), 24, 48 and 72 hours atl=365.5 nm, P= 125 W, g - irradiation with 60Co at doses 0 (control), 10, 20 and 40 Gy. Cultivars responded differentially to various doses of irradiation in both types of irradiation. Daidzein and genistein are the main isoflavones of biological interest belonging to the group of naturally occurring isoflavonoid phytoestrogens. Daidzein (Dai) and genistein (Gen) were determinated by a selective and sensitive radioimmunoassay 7- and 4´- cross-reactive method. In less amounts was determined biochanin A (Bio A) by means of 7-cross-reactivity. The highest levels among isoflavonoids were found for daidzein-7 cross-reactivity in both pea varieties (in average in Lantra seeds 6 562.4 mg kg-1 DM, in Menhir seeds 3 481.3 mg kg-1 DM). During spr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Agriculturae Bohemica

ISSN

0582-2343

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

14

Strana od-do

183-196

Kód UT WoS článku

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EID výsledku v databázi Scopus

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