Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005585" target="_blank" >RIV/60460709:41210/03:00005585 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil

Popis výsledku v původním jazyce

The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0,02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to thestage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. The amount of calcium deposits in the oocyte and granulose cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria, vacules, and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase of calcium deposits in the oocyte nucleus was dalayed. A much larger amount of deposits was demonstratively smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 hours of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb

Název v anglickém jazyce

Ultrastruktural Localization of Calcium Deposits in Pig Oocytes Maturing in Vitro: Effects of Verapamil

Popis výsledku anglicky

The culture of pig oocytes in the presence of the calcium channel blocker verapamil (0,02 mM) resulted in the blocking of meiosis at the metaphase I stage, and only a small fraction (about 28%) of the oocytes were able to continue their maturation to thestage of metaphase II. Hence, meiotic maturation in pig oocytes is a calcium-dependent process. The amount of calcium deposits in the oocyte and granulose cells increased during oocyte meiotic maturation in vitro, especially in the nucleus, mitochondria, vacules, and cytoplasm. The replenishment of calcium deposits is significantly changed under the effect of verapamil. The increase of calcium deposits in the oocyte nucleus was dalayed. A much larger amount of deposits was demonstratively smaller. A significant peak in the accumulation of calcium deposits was observed in the cytoplasm of verapamil-treated oocytes after 16 hours of in vitro culture. We propose that an altered pattern in the replenishment of calcium deposits can disturb

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

253-260

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—