Preparation of fowl testes as recipient organs to germ-line chimeras by means of gamma-radiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005704" target="_blank" >RIV/60460709:41210/03:00005704 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of fowl testes as recipient organs to germ-line chimeras by means of gamma-radiation

Popis výsledku v původním jazyce

The aim the study was to investigate the possibility of preparing adult fowl testes for the production of exogenous germ-lines by eradication of recipient spermatogenesis using gammaradiation A comparison between several radiation theraphy treatments (based on Co-60 isotope) of male testes was conducted using gamma-rays of 18, 22 and 26 Gy in a single dose or repeated doses of 5 x 8Gy over a 15-d period. Sperm concetration and motility were determined after each treatment Altered spermatogenesis was observed after a single treatment dose of 18 Gy, while single doses of 26 Gy were followed by reduced sperm numbers (from 22 x 10(9) to 31 x 10(6) sperm/ml) within 60 to 100 d after treatment. After a single treatment of 26 Gy sperm motility was reduced by50 %. In contrast, a fractionated treatment (5 x 8 Gy) with gamma- razs halted spermatogenesis 39 d after the distribution of the first 8 Gy dose Observations of the seminiferous tubules by electron microscopy performed 12 months after th

Název v anglickém jazyce

Preparation of fowl testes as recipient organs to germ-line chimeras by means of gamma-radiation

Popis výsledku anglicky

The aim the study was to investigate the possibility of preparing adult fowl testes for the production of exogenous germ-lines by eradication of recipient spermatogenesis using gammaradiation A comparison between several radiation theraphy treatments (based on Co-60 isotope) of male testes was conducted using gamma-rays of 18, 22 and 26 Gy in a single dose or repeated doses of 5 x 8Gy over a 15-d period. Sperm concetration and motility were determined after each treatment Altered spermatogenesis was observed after a single treatment dose of 18 Gy, while single doses of 26 Gy were followed by reduced sperm numbers (from 22 x 10(9) to 31 x 10(6) sperm/ml) within 60 to 100 d after treatment. After a single treatment of 26 Gy sperm motility was reduced by50 %. In contrast, a fractionated treatment (5 x 8 Gy) with gamma- razs halted spermatogenesis 39 d after the distribution of the first 8 Gy dose Observations of the seminiferous tubules by electron microscopy performed 12 months after th

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

British Poultry Science

ISSN

0007-1668

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

643-650

Kód UT WoS článku

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EID výsledku v databázi Scopus

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