Analýza cytokinů, které jsou zapojeny do růstu a samovolné regrese sarkomu u potkana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F07%3A23268" target="_blank" >RIV/60460709:41210/07:23268 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/07:00089246 RIV/67985904:_____/07:00089246

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Multiplex Analysis of Cytokines Involved in Tumour Growth and Spontaneous Regression in a Rat Sarcoma Model

Popis výsledku v původním jazyce

The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat-derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma,the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, other three cytokines(TNF-á, â-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.

Název v anglickém jazyce

Multiplex Analysis of Cytokines Involved in Tumour Growth and Spontaneous Regression in a Rat Sarcoma Model

Popis výsledku anglicky

The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat-derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma,the R5-28 cells were cultivated in vitro and then both the supernatants and the cell lysates were analysed. Our experiments showed three cytokines (MCP-1, TIMP-1 and VEGF) to be produced by R5-28 cells in vitro. Moreover, in vivo, other three cytokines(TNF-á, â-NGF and LIX) were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica - Prague

ISSN

0015-5500

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

53

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

216-219

Kód UT WoS článku

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EID výsledku v databázi Scopus

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