Immunochemical and HPLC identification of isoflavonoids in the Apiaceae family

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F12%3A57823" target="_blank" >RIV/60460709:41210/12:57823 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/12:43894002

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunochemical and HPLC identification of isoflavonoids in the Apiaceae family

Popis výsledku v původním jazyce

Ethanolic extracts of 23 species of the Apiaceae family were analyzed for their flavonoid and isoflavonoid content, using the following methods: high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS); semi-preparative HPLC with Enzyme Linked Immunosorbent Assay (HPLC-ELISA); and immunoaffinity extraction (IAE). Rutin and quercetin-3-glucoside were the most abundant flavonoid glycosides and the most abundant flavonoid aglycone was apigenin. The isoflavonoids daidzein, genistein,sissotrin and formononetin were also found. The estimated content of individual compounds ranged from 1 to over 800 mg/kg (dry weight) for flavonoids, and from 0.2 to over 60 mg/kg for isoflavonoids. The probable reason why isoflavonoids have rarely beenreported in the Apiaceae previously is that they occur in far smaller quantities than in families usually associated with isoflavonoids, such as the Leguminosae, but the sensitive methods used made their detection possible.

Název v anglickém jazyce

Immunochemical and HPLC identification of isoflavonoids in the Apiaceae family

Popis výsledku anglicky

Ethanolic extracts of 23 species of the Apiaceae family were analyzed for their flavonoid and isoflavonoid content, using the following methods: high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS); semi-preparative HPLC with Enzyme Linked Immunosorbent Assay (HPLC-ELISA); and immunoaffinity extraction (IAE). Rutin and quercetin-3-glucoside were the most abundant flavonoid glycosides and the most abundant flavonoid aglycone was apigenin. The isoflavonoids daidzein, genistein,sissotrin and formononetin were also found. The estimated content of individual compounds ranged from 1 to over 800 mg/kg (dry weight) for flavonoids, and from 0.2 to over 60 mg/kg for isoflavonoids. The probable reason why isoflavonoids have rarely beenreported in the Apiaceae previously is that they occur in far smaller quantities than in families usually associated with isoflavonoids, such as the Leguminosae, but the sensitive methods used made their detection possible.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F09%2F0994" target="_blank" >GA525/09/0994: Chemotaxonomie isoflavonoidů</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Systematics and Ecology

ISSN

0305-1978

e-ISSN

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Svazek periodika

2012

Číslo periodika v rámci svazku

45

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

237-243

Kód UT WoS článku

000311018100042

EID výsledku v databázi Scopus

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