Effect of hydrolyzed milk on the adhesion of lactobacilli to intestinal cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F15%3A67176" target="_blank" >RIV/60460709:41210/15:67176 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1515/sab-2015-0012" target="_blank" >http://dx.doi.org/10.1515/sab-2015-0012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/sab-2015-0012" target="_blank" >10.1515/sab-2015-0012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of hydrolyzed milk on the adhesion of lactobacilli to intestinal cells

Popis výsledku v původním jazyce

Milk is an essential part of the human diet and is undoubtedly a major calcium source in human nutrition, accepted well by most individuals. Knowledge on how the components from dairy products support or reduce the adherence of probiotics to the intestinal epithelium is limited. The purpose of this study was to investigate the effect of acid-hydrolyzed milk on the adhesion ability of two potentially probiotic strains (Lactobacillus plantarum S2, Lactobacillus gasseri R) to in vitro human intestinal epithelial model consisting of Caco-2 and mucus-secreting HT29-MTX co-culture. The adhesion of our tested strains L. gasseri and L. plantarum was 4,74 and 7,16%, respectively, when using inoculum of 2 * 108 CFU ml?1. Addition of acid-hydrolyzed milk to co-culture decreased the adherence by 53,7% for L. gasseri R and by 62,2% for L. plantarum S2. The results of this study evidently indicate the potential importance of the food matrix as a factor influencing probiotic colonization of the gut.

Název v anglickém jazyce

Effect of hydrolyzed milk on the adhesion of lactobacilli to intestinal cells

Popis výsledku anglicky

Milk is an essential part of the human diet and is undoubtedly a major calcium source in human nutrition, accepted well by most individuals. Knowledge on how the components from dairy products support or reduce the adherence of probiotics to the intestinal epithelium is limited. The purpose of this study was to investigate the effect of acid-hydrolyzed milk on the adhesion ability of two potentially probiotic strains (Lactobacillus plantarum S2, Lactobacillus gasseri R) to in vitro human intestinal epithelial model consisting of Caco-2 and mucus-secreting HT29-MTX co-culture. The adhesion of our tested strains L. gasseri and L. plantarum was 4,74 and 7,16%, respectively, when using inoculum of 2 * 108 CFU ml?1. Addition of acid-hydrolyzed milk to co-culture decreased the adherence by 53,7% for L. gasseri R and by 62,2% for L. plantarum S2. The results of this study evidently indicate the potential importance of the food matrix as a factor influencing probiotic colonization of the gut.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia Agriculturae Bohemica

ISSN

1211-3174

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

21-25

Kód UT WoS článku

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EID výsledku v databázi Scopus

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