Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73659" target="_blank" >RIV/60460709:41210/17:73659 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17221/389/2016-CJFS" target="_blank" >http://dx.doi.org/10.17221/389/2016-CJFS</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/389/2016-CJFS" target="_blank" >10.17221/389/2016-CJFS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers

Popis výsledku v původním jazyce

The development of bioanalytical methods has become challenging due to sample complexity, requirements for method reliability, and speed of analysis with triple quadrupole LC-MS/MS used widely for the routine analysis of biological materials. The article presents the method development and validation results for pelargonidin and malvidin in potato tubers. The developed method uses a short C18 column, is able to measure all six common anthocyanidins, uses a binary mobile phase with acetonitrile and water both with added 1 per cent formic acid, ESI ionisation in positive mode,three hour hydrolysis with 2,7 M methanolic HCl at 90 degrees C. For pelargonidin and malvidin, the method shows high recovery of 98 to 100 per cent, intraday repeatability of 6,7 to 17,9 per cent (depending on the analyte and concentration level), uncertainty below 20 per cent, and uses quadratic calibration.

Název v anglickém jazyce

Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers

Popis výsledku anglicky

The development of bioanalytical methods has become challenging due to sample complexity, requirements for method reliability, and speed of analysis with triple quadrupole LC-MS/MS used widely for the routine analysis of biological materials. The article presents the method development and validation results for pelargonidin and malvidin in potato tubers. The developed method uses a short C18 column, is able to measure all six common anthocyanidins, uses a binary mobile phase with acetonitrile and water both with added 1 per cent formic acid, ESI ionisation in positive mode,three hour hydrolysis with 2,7 M methanolic HCl at 90 degrees C. For pelargonidin and malvidin, the method shows high recovery of 98 to 100 per cent, intraday repeatability of 6,7 to 17,9 per cent (depending on the analyte and concentration level), uncertainty below 20 per cent, and uses quadratic calibration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Journal of Food Sciences

ISSN

1212-1800

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

223-228

Kód UT WoS článku

000404566800006

EID výsledku v databázi Scopus

2-s2.0-85021663828