Comparison of minidogfiler and “ASCH” STR multiplex systems for preliminary estimation of variability within wolf´s like dog breeds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A80828" target="_blank" >RIV/60460709:41210/19:80828 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1875176819304019?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1875176819304019?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsigss.2019.11.002" target="_blank" >10.1016/j.fsigss.2019.11.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of minidogfiler and “ASCH” STR multiplex systems for preliminary estimation of variability within wolf´s like dog breeds

Popis výsledku v původním jazyce

This study was undertaken to estimate basic population parameters and compare resolution capabilities of two chosen STR panels (Mini-DogFiler, MDF, ASCH). Wolfs like dog breeds, namely Czechoslovakian wolfdog (n = 55), Saarloos wolfdog (n = 22), and samples of the Eurasian wolf (n = 21) were selected for their close genetic relationship. Also, German Shepherd (n = 26) breed was included as a donor of dog genome for the Czechoslovakian wolfdog. Overall 228 alleles were detected using 20 STR markers (MDF 11 and ASCH 9 loci). Number of alleles ranged from 3 to 23 per locus, with median 11 alleles per locus for MDF and 10 for ASCH. Both panels showed similar level of variability in the means of number of alleles per locus (k) and observed heterozygostity (HO) as there were not found statistically significant differences (Mann-Whithey U-test, p = 0,85 for k, p = 0,88 for HO). A group of wolves showed the highest variability unlike Saarloos wolfdog with very low variability within all measured parameters,

Název v anglickém jazyce

Comparison of minidogfiler and “ASCH” STR multiplex systems for preliminary estimation of variability within wolf´s like dog breeds

Popis výsledku anglicky

This study was undertaken to estimate basic population parameters and compare resolution capabilities of two chosen STR panels (Mini-DogFiler, MDF, ASCH). Wolfs like dog breeds, namely Czechoslovakian wolfdog (n = 55), Saarloos wolfdog (n = 22), and samples of the Eurasian wolf (n = 21) were selected for their close genetic relationship. Also, German Shepherd (n = 26) breed was included as a donor of dog genome for the Czechoslovakian wolfdog. Overall 228 alleles were detected using 20 STR markers (MDF 11 and ASCH 9 loci). Number of alleles ranged from 3 to 23 per locus, with median 11 alleles per locus for MDF and 10 for ASCH. Both panels showed similar level of variability in the means of number of alleles per locus (k) and observed heterozygostity (HO) as there were not found statistically significant differences (Mann-Whithey U-test, p = 0,85 for k, p = 0,88 for HO). A group of wolves showed the highest variability unlike Saarloos wolfdog with very low variability within all measured parameters,

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30501 - Forensic science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Science International: Genetics Supplement Series

ISSN

1875-1768

e-ISSN

1875-175X

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

862-863

Kód UT WoS článku

000508217000333

EID výsledku v databázi Scopus

2-s2.0-85075854543