Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F21%3A88409" target="_blank" >RIV/60460709:41210/21:88409 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=226_2020-CJAS" target="_blank" >https://www.agriculturejournals.cz/web/cjas.htm?type=article&id=226_2020-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/226/2020-CJAS" target="_blank" >10.17221/226/2020-CJAS</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
Popis výsledku v původním jazyce
The aim of the present study was to optimize the conventional method of sperm freezing in liquid nitrogen LN2 vapour for successful cryopreservation of Wallachian ram sperm, the genetic resources of the Czech Republic. Sperm in straws were frozen using the conventional freezing method via a static exposure of sperm doses to LN2 vapour, or by four different modified freezing methods. Under modified freezing, straws were frozen by a discontinuous, time-dependent decremental change in the distance between the straws and the surface of LN 2. The viability of sperm was evaluated by flow cytometry after sperm equilibration, and immediately after thawing. Besides the observed inter-sire and daily variation, the obtained results suggest the methodological weakness of the conventional freezing method via the static exposure of sperm doses to LN 2 vapour. With the use of the optimized freezing procedure, all parameters of thawed sperm were significantly improved in comparison with the conventio
Název v anglickém jazyce
Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program
Popis výsledku anglicky
The aim of the present study was to optimize the conventional method of sperm freezing in liquid nitrogen LN2 vapour for successful cryopreservation of Wallachian ram sperm, the genetic resources of the Czech Republic. Sperm in straws were frozen using the conventional freezing method via a static exposure of sperm doses to LN2 vapour, or by four different modified freezing methods. Under modified freezing, straws were frozen by a discontinuous, time-dependent decremental change in the distance between the straws and the surface of LN 2. The viability of sperm was evaluated by flow cytometry after sperm equilibration, and immediately after thawing. Besides the observed inter-sire and daily variation, the obtained results suggest the methodological weakness of the conventional freezing method via the static exposure of sperm doses to LN 2 vapour. With the use of the optimized freezing procedure, all parameters of thawed sperm were significantly improved in comparison with the conventio
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40101 - Agriculture
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1805-9309
Svazek periodika
66
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
10
Strana od-do
55-64
Kód UT WoS článku
000620367500003
EID výsledku v databázi Scopus
2-s2.0-85102839144