Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F24%3A100953" target="_blank" >RIV/60460709:41210/24:100953 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2306-7381/11/12/602" target="_blank" >https://www.mdpi.com/2306-7381/11/12/602</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/vetsci11120602" target="_blank" >10.3390/vetsci11120602</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Popis výsledku v původním jazyce

It is well known that the suboptimal thawing of frozen insemination doses critically reduces the fertilizing ability of spermatozoa and, consequently, the effectiveness of artificial insemination. In this study, we aimed to determine the optimal thawing procedure for frozen Wallachian ram insemination doses. The study used a standard method of obtaining ejaculates from rams, diluting semen with a commercial extender, and a common methodology for freezing sperm in straws with minor modifications. After storage in liquid nitrogen, doses were thawed using several regimes: straws were thawed at 45, 50, 55, 60, and 65 degrees C for durations of 2, 5, 8, 11, and 14 s for each temperature. The thawing regime at 39 degrees for 30 s was chosen as a reference thawing method, to which all others were compared. Using a computer-assisted sperm analyser and flow cytometry, the motility, kinematic parameters, and spermatozoa cell organelle damage of the thawed samples were analysed and supplemented with an analysis of the internal temperature gradient and thermal energy absorption of each straw. Based on the obtained results, we concluded that the thawing regime at 39 degrees for 30 s is the preferred regime for ram sperm. Our study may be useful with regard to standardizing the procedure for the thawing of frozen ram sperm insemination doses and, therefore, might significantly improve the effectiveness of artificial insemination techniques in the sheep industry.

Název v anglickém jazyce

Effect of Different Thawing Regimes on Cell Kinematics and Organelle Integrity of Nitrogen-Stored Wallachian Ram Spermatozoa

Popis výsledku anglicky

It is well known that the suboptimal thawing of frozen insemination doses critically reduces the fertilizing ability of spermatozoa and, consequently, the effectiveness of artificial insemination. In this study, we aimed to determine the optimal thawing procedure for frozen Wallachian ram insemination doses. The study used a standard method of obtaining ejaculates from rams, diluting semen with a commercial extender, and a common methodology for freezing sperm in straws with minor modifications. After storage in liquid nitrogen, doses were thawed using several regimes: straws were thawed at 45, 50, 55, 60, and 65 degrees C for durations of 2, 5, 8, 11, and 14 s for each temperature. The thawing regime at 39 degrees for 30 s was chosen as a reference thawing method, to which all others were compared. Using a computer-assisted sperm analyser and flow cytometry, the motility, kinematic parameters, and spermatozoa cell organelle damage of the thawed samples were analysed and supplemented with an analysis of the internal temperature gradient and thermal energy absorption of each straw. Based on the obtained results, we concluded that the thawing regime at 39 degrees for 30 s is the preferred regime for ram sperm. Our study may be useful with regard to standardizing the procedure for the thawing of frozen ram sperm insemination doses and, therefore, might significantly improve the effectiveness of artificial insemination techniques in the sheep industry.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40200 - Animal and Dairy science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Sciences

ISSN

2306-7381

e-ISSN

2306-7381

Svazek periodika

11

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

1-13

Kód UT WoS článku

001384508600001

EID výsledku v databázi Scopus

2-s2.0-85213476315